Key features and details
- Mouse monoclonal  to MCP3 - BSA and Azide free
- Suitable for: ICC, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-MCP3 antibody  - BSA and Azide free
See all MCP3 primary antibodies
DescriptionMouse monoclonal  to MCP3 - BSA and Azide free
Tested applicationsSuitable for: ICC, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human MCP3 aa 24-99. Full-length mature chain. Genbank: NP_006264.2
Database link: P80098
- Flow cyt: THP-1 cells. ICC: SKBR3 cells.
Storage instructionsShipped at 4°C. Store at +4°C.
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab277128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/20 - 1/100.|
|Flow Cyt||1/25 - 1/100.|
FunctionChemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
- Information by UniProt
- C-C motif chemokine 7 antibody
- Ccl7 antibody
- CCL7_HUMAN antibody
Flow cytometric analysis of CCL7 expression on THP-1 (human monocytic leukemia cell line) cells. The cells were stained with ab277128 at 1/25 dilution, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence staining of Human CCL7 in SKBR3 (human mammary gland adenocarcinoma cell line) cells. Cells were fixed with 4% PFA, permeabilized with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab277128 at 1/60 dilution. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), counterstained with DAPI (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab277128 has not yet been referenced specifically in any publications.