Product nameAnti-MCP4 antibody (Biotin)
See all MCP4 primary antibodies
DescriptionGoat polyclonal to MCP4 (Biotin)
Tested applicationsSuitable for: WB, ELISAmore details
Predicted to work with: Human
- WB: Recombinant human MCP4 protein.
FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferConstituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa.|
|ELISA||Use a concentration of 0.25 - 1 µg/ml.
Human MCP-4 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271198. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 2000-4000 pg/ml of recombinant human MCP-4 protein.
FunctionChemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.
Tissue specificityWidely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
modificationsOne major form (form long), and two minor forms (short chain and medium chain) are produced by differential signal peptide cleavage. The medium chain is about 30-fold less active than the long chain.
- Information by UniProt
- C-C motif chemokine 13 antibody
- CCL13 antibody
- CCL13_HUMAN antibody
To detect Human MCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml.
When used in conjunction with compatible development reagents the detection limit for Recombinant Human MCP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human MCP4, respectively.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271198 has not yet been referenced specifically in any publications.