Product nameAnti-MCPH1 antibody
DescriptionRabbit polyclonal to MCPH1
Tested applicationsSuitable for: IP, WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human MCPH1 aa 555-701.
Database link: Q8NEM0
- Human cerebral cortex RT-4 cell lysate U-251 MG cell lysate
This product was previously labelled as BRIT1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionImplicated in chromosome condensation and DNA damage induced cellular responses. May play a role in neurogenesis and regulation of the size of the cerebral cortex.
Tissue specificityExpressed in fetal brain, liver and kidney.
Involvement in diseaseDefects in MCPH1 are the cause of microcephaly primary type 1 (MCPH1) [MIM:251200]; also known as true microcephaly or microcephaly vera. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. This entity is inherited as autosomal recessive trait.
Sequence similaritiesContains 3 BRCT domains.
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- BRCT repeat inhibitor of TERT expression 1 antibody
- BRIT 1 antibody
- FLJ12847 antibody
All lanes : Anti-MCPH1 antibody (ab121277) at 1/250 dilution
Lane 1 : Markers (kDa)
Lane 2 : RT-4 cell lysate
Lane 3 : U-251 MG cell lysate
Lane 4 : Human plasma
Lane 5 : Liver tissue lysate
Lane 6 : Tonsil tissue lysate
All lanes : HPR conjugated anti-rabbit antibody at 1/3000 dilution
Developed using the ECL technique.
ab121277, at 1/75, staining MCPH1 in neuronal cells of paraffin embedded Human cerebral cortex by Immunohistochemistry.
Immunohistochemical staining of Human testis shows strong positivity in a subset of cells in seminiferus ducts using ab121277
ab121277 has been referenced in 3 publications.
- Liu L et al. Silencing BRIT1 Facilitates the Abilities of Invasiveness and Migration in Trophoblast Cells. Med Sci Monit 24:7451-7458 (2018). PubMed: 30337515
- Ge C et al. The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex. PLoS One 10:e0144957 (2015). PubMed: 26683461
- Ge C et al. BRUCE regulates DNA double-strand break response by promoting USP8 deubiquitination of BRIT1. Proc Natl Acad Sci U S A 112:E1210-9 (2015). PubMed: 25733871