Key features and details
- Rabbit polyclonal to MCPIP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MCPIP1 antibody
See all MCPIP1 primary antibodies
DescriptionRabbit polyclonal to MCPIP1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment, corresponding to a sequence within amino acids 291-474 of Human MCPIP1 (NP_079355).
- WB: HEK-293T, A431,whole cell lysate. IHC-P: Mouse kidney tissue. IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 66 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionHas RNase activity and selectively degrades specific target mRNA species. Modulates the immune response and inflammation by regulating the decay of specific mRNA molecules. Recognizes the 3'-untranslated region (UTR) of the mRNA for IL6, CALCR and IL12B. Required for normal decay of IL6 mRNA (By similarity). Triggers apoptosis and promotes angiogenesis in response to the binding of CCL2 to CCR2. Regulates expression of CDH12 and CHD19.
Sequence similaritiesBelongs to the ZC3H12 family.
Contains 1 C3H1-type zinc finger.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- dJ423B22.1 antibody
- MCP 1 treatment-induced protein antibody
- MCP-induced protein 1 antibody
All lanes : Anti-MCPIP1 antibody (ab97910) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 66 kDa
Paraffin-embedded mouse kidney tissue stained for MCPIP1 at cytoplasm and nucleus using ab97910 at 1/250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunofluorescence analysis of paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling MCPIP1 protein (green) using ab97910 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
ab97910 has been referenced in 1 publication.
- Xue M et al. Up-regulated MCPIP1 in abdominal aortic aneurysm is associated with vascular smooth muscle cell apoptosis and MMPs production. Biosci Rep 39:N/A (2019). PubMed: 31651935