Anti-MCPT4 antibody (ab92368)

Overview

  • Product name
    Anti-MCPT4 antibody
  • Description
    Goat polyclonal to MCPT4
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity

    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    KLQKKAKETPSVN

    by a Cysteine residue linker, corresponding to internal sequence amino acids 114 - 126 of Mouse MCPT4

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab92368 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Has chymotrypsin-like activity. Hydrolyzes the amide bonds of synthetic substrates having Tyr and Phe residues at the P1 position. Preferentially hydrolyzes the 'Tyr-4-
    -Ile-5' bond of angiotensin I and the 'Phe-20-
    -Ala-21' bond of amyloid beta-protein, and is less active towards the 'Phe-8-
    -His-9' bond of angiotensin I and the 'Phe-4-
    -Ala-5' and 'Tyr-10-
    -Glu-11' bonds of amyloid beta-protein. Involved in thrombin regulation and fibronectin processing.
  • Tissue specificity
    Submucosal mast cells. In femoral muscle, detected in myocytes but not in mast cells.
  • Sequence similarities
    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mast cell protease 4 antibody
    • Mcp 4 antibody
    • Mcpt4 antibody
    • MCPT4_MOUSE antibody
    • MMCP 4 antibody
    • MMCP 4A antibody
    • MMCP 4B antibody
    • MMCP-4 antibody
    • MMCP4 antibody
    • MMCP4A antibody
    • MMCP4B antibody
    • MSMCP antibody
    • Myonase antibody
    • Serosal mast cell protease antibody
    see all

Images

  • Immunohistochemical staining of paraffin embedded Mouse Skeletal Muscle with ab92368 (3.75 µg/ml) . Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

ab92368 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Permeabilization
No
Fixative
10% Formalin
Username

Abcam user community

Verified customer

Submitted Aug 26 2014

Thank you for contacting us.

Please be aware that our ELISA assay is used for determining the activity of the antibody by applying serially diluted antibody to micro-wells that are coated with the immunizing peptide.

ELISA titre deter...

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Thank you for contacting us.

ab92368 is a Fast Track antibody. Fast-Track antibodies constitute a diverse group of products that have undergone variable degrees of characterization. They have all been affinity purified and show high titre valu...

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Thank you for contacting us regarding ab92368. As you may know, ab92368 is one of our Fast-track antibodies and it is not yet fully characterized. We cannot guarantee that the antibody will work in any application other than ELISA against the immun...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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