Anti-MCPyV_gp3 large T antigen antibody [Ab3] (ab202866)

Overview

  • Product name

    Anti-MCPyV_gp3 large T antigen antibody [Ab3]
  • Description

    Mouse monoclonal [Ab3] to MCPyV_gp3 large T antigen
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Immunogen

    Recombinant fragment (GST-tag) within MCPyV_gp3 large T antigen aa 1-300 (N terminal). The exact sequence is proprietary.

  • Epitope

    The epitope for monoclonal Ab3 is between amino acid residues 79 and 260 of MCPyV large T antigen. Ab3 does not bind to MCPyV small T antigen (PubMed ID: 23114601).
  • Positive control

    • WB: MKL-1 whole cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    Ab3
  • Isotype

    IgG1
  • Light chain type

    kappa

Applications

Our Abpromise guarantee covers the use of ab202866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 48 kDa.

Images

  • All lanes : Anti-MCPyV_gp3 large T antigen antibody [Ab3] (ab202866) at 0.5 µg/ml

    Lane 1 : MKL-1 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HEK293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Observed band size: 48 kDa
    why is the actual band size different from the predicted?



    This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab202866 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 0.5ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

References

This product has been referenced in:

  • Moshiri AS  et al. Polyomavirus-Negative Merkel Cell Carcinoma: A More Aggressive Subtype Based on Analysis of 282 Cases Using Multimodal Tumor Virus Detection. J Invest Dermatol 137:819-827 (2017). IHC-P . Read more (PubMed: 27815175) »
  • Spurgeon ME  et al. Tumorigenic activity of merkel cell polyomavirus T antigens expressed in the stratified epithelium of mice. Cancer Res 75:1068-79 (2015). WB . Read more (PubMed: 25596282) »
See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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