Key features and details
- Mouse monoclonal [Ab3] to MCPyV_gp3 large T antigen
- Suitable for: WB, ICC/IF
- Isotype: IgG1
Product nameAnti-MCPyV_gp3 large T antigen antibody [Ab3]
See all MCPyV_gp3 large T antigen primary antibodies
DescriptionMouse monoclonal [Ab3] to MCPyV_gp3 large T antigen
Tested applicationsSuitable for: WB, ICC/IFmore details
Recombinant fragment (GST-tag) within MCPyV_gp3 large T antigen aa 1-300 (N terminal). The exact sequence is proprietary.
EpitopeThe epitope for monoclonal Ab3 is between amino acid residues 79 and 260 of MCPyV large T antigen. Ab3 does not bind to MCPyV small T antigen (PubMed ID: 23114601).
- WB: MKL-1 whole cell lysate. ICC: MKL-1 cells
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact firstname.lastname@example.org.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab202866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 48 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
All lanes : Anti-MCPyV_gp3 large T antigen antibody [Ab3] (ab202866) at 0.5 µg/ml
Lane 1 : MKL-1 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HEK293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 48 kDa why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab202866 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 0.5ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
ab202866 staining MCPyV_gp3 large T antigen in MKL-1 cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202866 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab202866 has been referenced in 5 publications.
- Moshiri AS et al. Polyomavirus-Negative Merkel Cell Carcinoma: A More Aggressive Subtype Based on Analysis of 282 Cases Using Multimodal Tumor Virus Detection. J Invest Dermatol 137:819-827 (2017). IHC-P . PubMed: 27815175
- Spurgeon ME et al. Tumorigenic activity of merkel cell polyomavirus T antigens expressed in the stratified epithelium of mice. Cancer Res 75:1068-79 (2015). WB . PubMed: 25596282
- Xie H et al. MicroRNA expression patterns related to merkel cell polyomavirus infection in human merkel cell carcinoma. J Invest Dermatol 134:507-517 (2014). IHC-P . PubMed: 23962809
- Cheng J et al. Merkel cell polyomavirus large T antigen has growth-promoting and inhibitory activities. J Virol 87:6118-26 (2013). WB . PubMed: 23514892
- Rodig SJ et al. Improved detection suggests all Merkel cell carcinomas harbor Merkel polyomavirus. J Clin Invest 122:4645-53 (2012). WB, IHC-P . PubMed: 23114601