Product nameAnti-MCPyV_gp3 large T antigen antibody [Ab3]
DescriptionMouse monoclonal [Ab3] to MCPyV_gp3 large T antigen
Tested applicationsSuitable for: WBmore details
Recombinant fragment (GST-tag) within MCPyV_gp3 large T antigen aa 1-300 (N terminal). The exact sequence is proprietary.
EpitopeThe epitope for monoclonal Ab3 is between amino acid residues 79 and 260 of MCPyV large T antigen. Ab3 does not bind to MCPyV small T antigen (PubMed ID: 23114601).
- WB: MKL-1 whole cell lysate.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab202866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 48 kDa.|
All lanes : Anti-MCPyV_gp3 large T antigen antibody [Ab3] (ab202866) at 0.5 µg/ml
Lane 1 : MKL-1 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HEK293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 48 kDa why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab202866 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 0.5ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This product has been referenced in:
- Moshiri AS et al. Polyomavirus-Negative Merkel Cell Carcinoma: A More Aggressive Subtype Based on Analysis of 282 Cases Using Multimodal Tumor Virus Detection. J Invest Dermatol 137:819-827 (2017). IHC-P . Read more (PubMed: 27815175) »
- Spurgeon ME et al. Tumorigenic activity of merkel cell polyomavirus T antigens expressed in the stratified epithelium of mice. Cancer Res 75:1068-79 (2015). WB . Read more (PubMed: 25596282) »