Product nameAnti-MCPyV_gp3 large T antigen antibody [Ab3] - BSA and Azide free
See all MCPyV_gp3 large T antigen primary antibodies
DescriptionMouse monoclonal [Ab3] to MCPyV_gp3 large T antigen - BSA and Azide free
Tested applicationsSuitable for: WB, ICC/IFmore details
Recombinant fragment (GST-tag) within MCPyV_gp3 large T antigen aa 1-300 (N terminal). The exact sequence is proprietary.
EpitopeThe epitope for monoclonal Ab3 is between amino acid residues 79 and 260 of MCPyV large T antigen. Ab3 does not bind to MCPyV small T antigen (PubMed ID: 23114601).
- WB: MKL-1 whole cell lysate. ICC: MKL-1 cells
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact email@example.com.
ab269582 is the carrier-free version of ab202866. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab269582 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab269582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 48 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
All lanes : Anti-MCPyV_gp3 large T antigen antibody [Ab3] (ab202866) at 0.5 µg/ml
Lane 1 : MKL-1 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HEK293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab202866 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 0.5µg/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This image was produced using the same antibody clone but in a different formulation ab202866, PBS and sodium azide.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab202866)
ab202866 staining MCPyV_gp3 large T antigen in MKL-1 cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202866 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab269582 has not yet been referenced specifically in any publications.