Anti-MCRS1/MSP58 antibody (ab247013)
Key features and details
- Rabbit polyclonal to MCRS1/MSP58
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MCRS1/MSP58 antibody -
Description
Rabbit polyclonal to MCRS1/MSP58 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human MCRS1/MSP58 aa 200-350.
Database link: Q96EZ8 -
Positive control
- IHC-P: Human testis tissue. WB: NIH/3T3, NBT-II and RT4 cell lysate. ICC/IF: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. May be an inhibitor of TERT telomerase activity.
Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. -
Tissue specificity
Detected in testis, and at lower levels in spleen, thymus, prostate, uterus, small intestine, colon and leukocytes. -
Sequence similarities
Contains 1 FHA domain. -
Developmental stage
Cell-cycle regulated: levels are highest early in S phase; not detectable in G2. -
Cellular localization
Nucleus. Nucleus > nucleolus. In microspherules in the nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 10445 Human
- Entrez Gene: 51812 Mouse
- Entrez Gene: 300222 Rat
- Omim: 609504 Human
- SwissProt: Q96EZ8 Human
- SwissProt: Q99L90 Mouse
- Unigene: 25313 Human
- Unigene: 383472 Mouse
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Alternative names
- 58 kDa microspherule protein antibody
- Cell cycle regulated factor (78 kDa) antibody
- Cell cycle regulated factor p78 antibody
see all
Images
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PFA fixed, Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling MCRS1/MSP58 using ab247013 at 4 µg/ml (green) in ICC/IF.
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Paraffin-embedded human testis tissue stained for MCRS1/MSP58 using ab247013 at 1/1000 dilution in immunohistochemical analysis.
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Anti-MCRS1/MSP58 antibody (ab247013) at 0.4 µg/ml + RT4 (Human urinary bladder cancer cell line) cell lysate
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All lanes : Anti-MCRS1/MSP58 antibody (ab247013) at 0.4 µg/ml
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II (Rat cell line) cell lysate
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab247013 has been referenced in 1 publication.
- Neilson KM et al. Mcrs1 interacts with Six1 to influence early craniofacial and otic development. Dev Biol 467:39-50 (2020). PubMed: 32891623