Anti-CSF-1-R antibody (ab223642)
Key features and details
- Goat polyclonal to CSF-1-R
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CSF-1-R antibody
See all CSF-1-R primary antibodies -
Description
Goat polyclonal to CSF-1-R -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cat -
Immunogen
Synthetic peptide corresponding to Human CSF-1-R aa 700-800 (internal sequence) (Cysteine residue). (NP_005202.2).
Database link: P07333 -
Positive control
- WB: HEK-293 lysate overexpressing human CSF-1-R with C-terminal MYC tag.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab223642 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 108 kDa.
A 1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 108 kDa. A 1 hour primary incubation is recommended for this product. |
Target
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Function
Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34. -
Tissue specificity
Expressed in bone marrow and in differentiated blood mononuclear cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 493706 Cat
- Entrez Gene: 1436 Human
- Entrez Gene: 12978 Mouse
- Entrez Gene: 307403 Rat
- Omim: 164770 Human
- SwissProt: P13369 Cat
- SwissProt: P07333 Human
- SwissProt: P09581 Mouse
see all -
Alternative names
- C FMS antibody
- CD 115 antibody
- CD115 antibody
see all
Images
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Lane 1 : Anti-CSF-1-R antibody (ab223642) at 0.5 µg/ml
Lane 2 : Anti-CSF-1-R antibody (ab223642) at 1 mg/ml
Lane 3 : Anti-MYC tag at 1/1000 dilution
Lanes 1 & 3 : HEK-293 (human epithelial cell line from embryonic kidney) lysate overexpressing human CSF-1-R with C-terminal MYC tag (in RIPA buffer)
Lane 2 : Mock transfected HEK-293 (human epithelial cell line from embryonic kidney) lysate (in RIPA buffer)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 108 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?Primary incubations were for 1 hour.
Datasheets and documents
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Datasheet download
References (0)
ab223642 has not yet been referenced specifically in any publications.