Recombinant
RabMAb

Recombinant Anti-MCSF Receptor antibody [EPR20754] (ab229188)

Overview

  • Product name

    Anti-MCSF Receptor antibody [EPR20754]
    See all MCSF Receptor primary antibodies
  • Description

    Rabbit monoclonal [EPR20754] to MCSF Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human MCSF Receptor aa 1-300. The exact sequence is proprietary.
    Database link: P07333

  • Positive control

    • WB: Human placenta and tonsil lysates. IHC-P: Human tonsil, non-Hodgkin lymphoma and lung cancer tissues. IP: Human tonsil lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab229188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).

Target

  • Function

    Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34.
  • Tissue specificity

    Expressed in bone marrow and in differentiated blood mononuclear cells.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C FMS antibody
    • CD 115 antibody
    • CD115 antibody
    • CD115 antigen antibody
    • CFMS antibody
    • Colony stimulating factor 1 receptor antibody
    • Colony stimulating factor I receptor antibody
    • CSF 1 R antibody
    • CSF 1R antibody
    • CSF-1 receptor antibody
    • CSF-1-R antibody
    • CSF1 R antibody
    • CSF1R antibody
    • CSF1R_HUMAN antibody
    • CSFR antibody
    • EC 2.7.10.1 antibody
    • FIM 2 antibody
    • FIM2 antibody
    • FMS antibody
    • FMS proto oncogene antibody
    • FMS protooncogene antibody
    • HDLS antibody
    • M-CSF Receptor antibody
    • M-CSF-R antibody
    • Macrophage colony stimulating factor 1 receptor antibody
    • Macrophage colony stimulating factor I receptor antibody
    • Macrophage colony-stimulating factor 1 receptor antibody
    • McDonough feline sarcoma viral (v fms) oncogene homolog antibody
    • MCSFR antibody
    • Oncogen FMS antibody
    • Proto-oncogene c-Fms antibody
    • V-FMS McDonough feline sarcoma viral oncogen homolog, formerly antibody
    see all

Images

  • All lanes : Anti-MCSF Receptor antibody [EPR20754] (ab229188) at 1/1000 dilution

    Lane 1 : Human placenta lysate
    Lane 2 : Human tonsil lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 108 kDa
    Observed band size: 108 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling MCSF Receptor with ab229188 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic staining in macrophages infiltrating human lung cancer is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma tissue labeling MCSF Receptor with ab229188 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic and membranous staining of human non-Hodgkin lymphoma cells is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MCSF Receptor with ab229188 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic staining in macrophages of human tonsil (PMID: 26066800) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • MCSF Receptor was immunoprecipitated from 0.35 mg of human tonsil lysate with ab229188 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229188 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human tonsil lysate 10 µg (Input). 

    Lane 2: ab229188 IP in human tonsil lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229188 in human tonsil lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

References

ab229188 has not yet been referenced specifically in any publications.

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