Overview

  • Product name
  • Description
    Goat polyclonal to MCT-1
  • Host species
    Goat
  • Specificity
    ab156807 is expected to recognize both reported isoforms (NP_054779.1; NP_001131026.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human MCT-1 aa 6-20 (N terminal).
    Sequence:

    DEKENVSNCIQLKTS


    Database link: NP_054779.1

  • Positive control
    • MOLT4 and Jurkat cell lysates.
  • General notes

    Previously labelled as Malignant T cell amplified sequence 1. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab156807 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 21 kDa).

Target

  • Function
    Anti-oncogene that play a role in cell cycle regulation; decreases cell doubling time and anchorage-dependent growth; shortens the duration of G1 transit time and G1/S transition. When constituvely expressed, increases CDK4 and CDK6 kinases activity and CCND1/cyclin D1 protein level, as well as G1 cyclin/CDK complex formation. Plays a role as translation enhancer; Recruits the density-regulated protein/DENR and binds to the cap complex of the 5'-terminus of mRNAs, subsequently altering the mRNA translation profile; Up-regulates protein levels of BCL2L2, TFDP1, MRE11A, CCND1 and E2F1, while mRNA levels remains constant. Hyperactivates DNA damage signaling pathway; increased gamma-irradiation-induced phosphorylation of histone H2AX, and induces damage foci formation. Increases the overall number of chromosomal abnormalities such as larger chromosomes formation and multiples chromosomal fusions when over-expressed in gamma-irradiated cells. May play a role in promoting lymphoid tumor development: lymphoid cell lines over-expressing MCTS1 exhibit increased growth rates and display increased protection against apoptosis. May contribute to the pathogenesis and progression of breast cancer via promotion of angiogenesis through the decline of inhibitory THBS1/thrombospondin-1, and inhibition of apoptosis. Involved in the process of proteasome degradation to down-regulate Tumor suppressor p53/TP53 in breast cancer cell; Positively regulates phosphorylation of MAPK1 and MAPK3.
  • Tissue specificity
    Ubiquitous. Over-expressed in T-cell lymphoid cell lines and in non-Hodgkin lymphoma cell lines as well as in a subset of primary large B-cell lymphomas.
  • Sequence similarities
    Belongs to the MCTS1 family.
    Contains 1 PUA domain.
  • Domain
    The PUA RNA-binding domain is critical for cap binding, but not sufficient for translation enhancer function. MCT1 N-terminal region is required to enhance translation possibly trough interaction with other proteins.
  • Post-translational
    modifications
    Phosphorylation is critical for stabilization and promotion of cell proliferation.
  • Cellular localization
    Cytoplasm. Nuclear relocalization after DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ39637 antibody
    • Malignant T cell amplified sequence 1 antibody
    • Malignant T cell-amplified sequence 1 antibody
    • MCT 1 antibody
    • MCT-1 antibody
    • MCT1 antibody
    • MCTS 1 antibody
    • MCTS1 antibody
    • MCTS1_HUMAN antibody
    • Multiple copies T cell malignancies 1 antibody
    • Multiple copies T cell malignancies antibody
    • Multiple copies T-cell malignancies antibody
    • Oncogene MCT 1 antibody
    • Oncogene MCT1 antibody
    see all

Images

  • Anti-MCT-1 antibody (ab156807) at 0.3 µg/ml + MOLT4 cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 21 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab156807 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab156807.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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