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    mct-1-antibody-ab156807.pdf

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Cancer Oncoproteins/suppressors Oncoproteins Other
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Anti-MCT-1 antibody (ab156807)

  • Datasheet
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Western blot - Anti-MCT-1 antibody (ab156807)

    Key features and details

    • Goat polyclonal to MCT-1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human MCT-1 protein (ab140541)
    Secondary
    Product image
    Donkey Anti-Goat IgG H&L (HRP) (ab97110)

    View more associated products

    Overview

    • Product name

      Anti-MCT-1 antibody
      See all MCT-1 primary antibodies
    • Description

      Goat polyclonal to MCT-1
    • Host species

      Goat
    • Specificity

      ab156807 is expected to recognize both reported isoforms (NP_054779.1; NP_001131026.1).
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
    • Immunogen

      Synthetic peptide corresponding to Human MCT-1 aa 6-20 (N terminal).
      Sequence:

      DEKENVSNCIQLKTS


      Database link: NP_054779.1
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • MOLT4 and Jurkat cell lysates.
    • General notes

      Previously labelled as Malignant T cell amplified sequence 1. 

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 99% Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab156807 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Other

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Positive Controls

      • Jurkat whole cell lysate (ab30128)
      • Jurkat whole cell lysate (ab7899)
      • MOLT-4 whole cell lysate (ab7912)
    • Recombinant Protein

      • Recombinant Human MCT-1 protein (ab140541)
    • Related Products

      • Recombinant Human MCT-1 protein (ab140541)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab156807 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 21 kDa).

    1 hour primary incubation is recommended for this product.

    Notes
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 21 kDa).

    1 hour primary incubation is recommended for this product.

    Target

    • Function

      Anti-oncogene that play a role in cell cycle regulation; decreases cell doubling time and anchorage-dependent growth; shortens the duration of G1 transit time and G1/S transition. When constituvely expressed, increases CDK4 and CDK6 kinases activity and CCND1/cyclin D1 protein level, as well as G1 cyclin/CDK complex formation. Plays a role as translation enhancer; Recruits the density-regulated protein/DENR and binds to the cap complex of the 5'-terminus of mRNAs, subsequently altering the mRNA translation profile; Up-regulates protein levels of BCL2L2, TFDP1, MRE11A, CCND1 and E2F1, while mRNA levels remains constant. Hyperactivates DNA damage signaling pathway; increased gamma-irradiation-induced phosphorylation of histone H2AX, and induces damage foci formation. Increases the overall number of chromosomal abnormalities such as larger chromosomes formation and multiples chromosomal fusions when over-expressed in gamma-irradiated cells. May play a role in promoting lymphoid tumor development: lymphoid cell lines over-expressing MCTS1 exhibit increased growth rates and display increased protection against apoptosis. May contribute to the pathogenesis and progression of breast cancer via promotion of angiogenesis through the decline of inhibitory THBS1/thrombospondin-1, and inhibition of apoptosis. Involved in the process of proteasome degradation to down-regulate Tumor suppressor p53/TP53 in breast cancer cell; Positively regulates phosphorylation of MAPK1 and MAPK3.
    • Tissue specificity

      Ubiquitous. Over-expressed in T-cell lymphoid cell lines and in non-Hodgkin lymphoma cell lines as well as in a subset of primary large B-cell lymphomas.
    • Sequence similarities

      Belongs to the MCTS1 family.
      Contains 1 PUA domain.
    • Domain

      The PUA RNA-binding domain is critical for cap binding, but not sufficient for translation enhancer function. MCT1 N-terminal region is required to enhance translation possibly trough interaction with other proteins.
    • Post-translational
      modifications

      Phosphorylation is critical for stabilization and promotion of cell proliferation.
    • Cellular localization

      Cytoplasm. Nuclear relocalization after DNA damage.
    • Target information above from: UniProt accession Q9ULC4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 422363 Chicken
      • Entrez Gene: 508412 Cow
      • Entrez Gene: 481038 Dog
      • Entrez Gene: 28985 Human
      • Entrez Gene: 68995 Mouse
      • Entrez Gene: 100511797 Pig
      • Entrez Gene: 302500 Rat
      • Entrez Gene: 448679 Xenopus tropicalis
      • Entrez Gene: 503679 Xenopus tropicalis
      • Entrez Gene: 394046 Zebrafish
      • Omim: 300587 Human
      • SwissProt: Q5ZI42 Chicken
      • SwissProt: Q2KIE4 Cow
      • SwissProt: Q9ULC4 Human
      • SwissProt: Q9DB27 Mouse
      • SwissProt: Q4G009 Rat
      • SwissProt: Q5PPY1 Xenopus laevis
      • SwissProt: Q6DER1 Xenopus tropicalis
      • SwissProt: Q7ZV34 Zebrafish
      • Unigene: 102696 Human
      • Unigene: 670803 Human
      • Unigene: 262453 Mouse
      • Unigene: 36129 Rat
      • Unigene: 84976 Zebrafish
      see all
    • Alternative names

      • FLJ39637 antibody
      • Malignant T cell amplified sequence 1 antibody
      • Malignant T cell-amplified sequence 1 antibody
      • MCT 1 antibody
      • MCT-1 antibody
      • MCT1 antibody
      • MCTS 1 antibody
      • MCTS1 antibody
      • MCTS1_HUMAN antibody
      • Multiple copies T cell malignancies 1 antibody
      • Multiple copies T cell malignancies antibody
      • Multiple copies T-cell malignancies antibody
      • Oncogene MCT 1 antibody
      • Oncogene MCT1 antibody
      see all

    Images

    • Western blot - Anti-MCT-1 antibody (ab156807)
      Western blot - Anti-MCT-1 antibody (ab156807)
      Anti-MCT-1 antibody (ab156807) at 0.3 µg/ml + MOLT4 cell lysate (in RIPA buffer) at 35 µg

      Developed using the ECL technique.

      Predicted band size: 21 kDa
      Observed band size: 19 kDa why is the actual band size different from the predicted?



      Primary incubation was 1 hour.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab156807? Please let us know so that we can cite the reference in this datasheet.

    ab156807 has not yet been referenced specifically in any publications.

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