Anti-MCT-1 antibody (ab156807)
Key features and details
- Goat polyclonal to MCT-1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MCT-1 antibody
See all MCT-1 primary antibodies -
Description
Goat polyclonal to MCT-1 -
Host species
Goat -
Specificity
ab156807 is expected to recognize both reported isoforms (NP_054779.1; NP_001131026.1). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
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Immunogen
Synthetic peptide corresponding to Human MCT-1 aa 6-20 (N terminal).
Sequence:DEKENVSNCIQLKTS
Database link: NP_054779.1 -
Positive control
- MOLT4 and Jurkat cell lysates.
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General notes
Previously labelled as Malignant T cell amplified sequence 1.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab156807 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 21 kDa).
1 hour primary incubation is recommended for this product. |
| Notes |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 21 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Anti-oncogene that play a role in cell cycle regulation; decreases cell doubling time and anchorage-dependent growth; shortens the duration of G1 transit time and G1/S transition. When constituvely expressed, increases CDK4 and CDK6 kinases activity and CCND1/cyclin D1 protein level, as well as G1 cyclin/CDK complex formation. Plays a role as translation enhancer; Recruits the density-regulated protein/DENR and binds to the cap complex of the 5'-terminus of mRNAs, subsequently altering the mRNA translation profile; Up-regulates protein levels of BCL2L2, TFDP1, MRE11A, CCND1 and E2F1, while mRNA levels remains constant. Hyperactivates DNA damage signaling pathway; increased gamma-irradiation-induced phosphorylation of histone H2AX, and induces damage foci formation. Increases the overall number of chromosomal abnormalities such as larger chromosomes formation and multiples chromosomal fusions when over-expressed in gamma-irradiated cells. May play a role in promoting lymphoid tumor development: lymphoid cell lines over-expressing MCTS1 exhibit increased growth rates and display increased protection against apoptosis. May contribute to the pathogenesis and progression of breast cancer via promotion of angiogenesis through the decline of inhibitory THBS1/thrombospondin-1, and inhibition of apoptosis. Involved in the process of proteasome degradation to down-regulate Tumor suppressor p53/TP53 in breast cancer cell; Positively regulates phosphorylation of MAPK1 and MAPK3. -
Tissue specificity
Ubiquitous. Over-expressed in T-cell lymphoid cell lines and in non-Hodgkin lymphoma cell lines as well as in a subset of primary large B-cell lymphomas. -
Sequence similarities
Belongs to the MCTS1 family.
Contains 1 PUA domain. -
Domain
The PUA RNA-binding domain is critical for cap binding, but not sufficient for translation enhancer function. MCT1 N-terminal region is required to enhance translation possibly trough interaction with other proteins. -
Post-translational
modificationsPhosphorylation is critical for stabilization and promotion of cell proliferation. -
Cellular localization
Cytoplasm. Nuclear relocalization after DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 422363 Chicken
- Entrez Gene: 508412 Cow
- Entrez Gene: 481038 Dog
- Entrez Gene: 28985 Human
- Entrez Gene: 68995 Mouse
- Entrez Gene: 100511797 Pig
- Entrez Gene: 302500 Rat
- Entrez Gene: 448679 Xenopus tropicalis
see all -
Alternative names
- FLJ39637 antibody
- Malignant T cell amplified sequence 1 antibody
- Malignant T cell-amplified sequence 1 antibody
see all
Images
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Western blot - Anti-MCT-1 antibody (ab156807)Anti-MCT-1 antibody (ab156807) at 0.3 µg/ml + MOLT4 cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab156807 has not yet been referenced specifically in any publications.