Overview

  • Product name

  • Description

    Goat polyclonal to MCT2
  • Host species

    Goat
  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Monkey, Gorilla, Common marmoset
  • Immunogen

    Synthetic peptide:

    SNAQSVTSERETNI

    with a Cysteine residue linker, corresponding to C terminal amino acids 465-478 of Human MCT2 (NP_004722.2).

  • Positive control

    • Human testis tissue; A549 lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab129290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/128000.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 52 kDa.
IHC-P Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. MCT2 is a high affinity pyruvate transporter.
  • Tissue specificity

    High expression in testis, moderate to low in spleen, heart, kidney, pancreas, skeletal muscle, brain and Leukocyte. Restricted expression in normal tissues, but widely expressed in cancer cells.
  • Sequence similarities

    Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • MCT 2 antibody
    • MCT antibody
    • Monocarboxylate transporter 2 antibody
    • MonocarboxylateTransporter 2 antibody
    • MOT2 antibody
    • MOT2_HUMAN antibody
    • SLC16A7 antibody
    • Solute carrier family 16 (monocarboxylate transporter), member 7 antibody
    • Solute carrier family 16 member 7 antibody
    • Solute carrier Family 16 Monocarboxylic Acid Transporters Member 7 antibody
    • Solute carrier family 16, member 7 (monocarboxylic acid transporter 2) antibody
    see all

Images

  • ab129290, at 3.75µg/ml, staining MCT2 in Formalin-fixed, Paraffin-embedded Human testis tissue by Immunohistochemistry.
  • Anti-MCT2 antibody (ab129290) at 1 µg/ml + A549 lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 52 kDa

References

This product has been referenced in:

  • Giatromanolaki A  et al. Programmed death-1 receptor (PD-1) and PD-ligand-1 (PD-L1) expression in non-small cell lung cancer and the immune-suppressive effect of anaerobic glycolysis. Med Oncol 36:76 (2019). Read more (PubMed: 31342270) »
  • Lund J  et al. Utilization of lactic acid in human myotubes and interplay with glucose and fatty acid metabolism. Sci Rep 8:9814 (2018). Read more (PubMed: 29959350) »
See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab129290.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up