Overview

  • Product name

  • Description

    Rabbit polyclonal to MCU
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment: HHRTVHQRIA SWQNLGAVYC STVVPSDDVT VVYQNGLPVI SVRLPSRRER CQFTLKPISD SVGVFLRQLQ EEDRGIDRVA IYSPDGVRVA ASTGIDLLLL DDFKLV, corresponding to N terminal amino acids 47-152 of Human MCU (Uniprot ID = Q8NE86)

  • Positive control

    • Human duodenum tissue. Human cell line U-2 OS.

Properties

Applications

Our Abpromise guarantee covers the use of ab121499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Recommended fixative: PFA/Triton X-100

Target

Images

  • ab121499 staining MCU in Human small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Image shows moderate to strong granular cytoplasmic positivity in glandular cells

     

  • All lanes : Anti-MCU antibody (ab121499) at 0.4 µg/ml

    Lane 1 : A-549 cell lysate transfected with control siRNA
    Lane 2 : A-549 cell lysate transfected with target specific siRNA probe #1
    Lane 3 : A-549 cell lysate transfected with target specific siRNA probe #2

    Predicted band size: 40 kDa



    Loading control, Anti-PPIB.

  • ab121499 staining MCU in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Image shows strong granular cytoplasmic positivity in glandular cells

  • Anti-MCU antibody (ab121499) + Human cell line A-549

    Predicted band size: 40 kDa

  • Immunofluorescent staining of Human cell line U-2 OS (fixed and permeabilized in PFA/Triton X-100) labeling MCU with ab121499 at 4µg/ml.
  • Immunohistochemical analysis of paraffin-embedded Human duodenum labeling MCU in glandular cells with ab121499 at 1/50 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 was performed.

References

This product has been referenced in:

  • Kim Y  et al. Protein composition of the muscle mitochondrial reticulum during postnatal development. J Physiol 597:2707-2727 (2019). Read more (PubMed: 30919448) »
  • Shamseldin HE  et al. A null mutation in MICU2 causes abnormal mitochondrial calcium homeostasis and a severe neurodevelopmental disorder. Brain 140:2806-2813 (2017). Read more (PubMed: 29053821) »
See all 8 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Spinal Cord)
Permeabilization
Yes - 0.3% TritonX-100
Specification
Spinal Cord
Blocking step
BSA + Normal Goat Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 07 2015

Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (4-12& BIS-TRIS)
Sample
Human Cell lysate - whole cell (fibroblasts)
Specification
fibroblasts
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Feb 20 2014

Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Heart)
Loading amount
30 µg
Specification
Heart
Treatment
miRNA overexpression
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 27°C

Dr. Samarjit Das

Verified customer

Submitted Apr 19 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up