Key features and details
- Mouse monoclonal [CL3576] to MCU
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-MCU antibody [CL3576]
DescriptionMouse monoclonal [CL3576] to MCU
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
EpitopeBinds to an epitope located within the peptide sequence RDRQYLLFFH as determined by overlapping synthetic peptides.
- A549 cell extracts; A549 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab219827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 2 - 10 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||1/500 - 1/1000. Predicted molecular weight: 40 kDa.|
Sequence similaritiesBelongs to the CCDC109 family.
- Information by UniProt
- C109A_HUMAN antibody
- C10orf42 antibody
- Calcium uniporter protein mitochondrial antibody
PFA-fixed, Triton X-100 permeabilized A549 cells stained for MCU (Green) using ab219827 at 4 μg/ml in ICC/IF.
Microtublues are stained red, nuclei are stained blue.
Anti-MCU antibody [CL3576] (ab219827) at 1/500 dilution + A549 cell extract
Predicted band size: 40 kDa
All lanes : Anti-MCU antibody [CL3576] (ab219827) at 1/500 dilution
Lane 1 : A549 transfected with control siRNA cell extract
Lane 2 : A549 transfected with target specific siRNA #1 cell extract
Lane 3 : A549 transfected with target specific siRNA #2 cell extract
Predicted band size: 40 kDa
Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative to the control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.
ab219827 has not yet been referenced specifically in any publications.