Anti-MD1 antibody (ab45424)

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Western blot - Anti-MD1 antibody (ab45424)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MD1 antibody (ab45424)

Key features and details

  • Rabbit polyclonal to MD1
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-MD1 antibody
    See all MD1 primary antibodies

  • Description

    Rabbit polyclonal to MD1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic 15 amino acid peptide from near the center of human MD1

  • Positive control

    • Daudi cell lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody is an immunoaffinity purified IgG

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab45424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 2.5 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 18 kDa).
Notes
IHC-P
Use a concentration of 2.5 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 18 kDa).

Target

  • Function

    May cooperate with CD180 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) and cytokine production. Important for efficient CD180 cell surface expression.

  • Tissue specificity

    Highly expressed in B-cells, monocytes and tonsil.

  • Cellular localization

    Secreted > extracellular space. Associated with CD180 at the cell surface.
  • Information by UniProt

  • Database links

    • Alternative names

      • BPAD antibody
      • dJ80N2.1 antibody
      • Ly 86 antibody
      • Ly-86 antibody
      • Ly86 antibody
      • LY86_HUMAN antibody
      • Lymphocyte antigen 86 antibody
      • Lymphocyte antigen 86 precursor antibody
      • Major affective disorder 1 antibody
      • MD 1 antibody
      • MD-1 antibody
      • MD1 protein antibody
      • MD1 RP105 associated antibody
      • MMD 1 antibody
      • MMD-1 antibody
      • Protein MD 1 antibody
      • Protein MD-1 antibody
      • RP1-80N2.1 antibody
      see all

    Images

    • Western blot - Anti-MD1 antibody (ab45424)
      Western blot - Anti-MD1 antibody (ab45424)
      Anti-MD1 antibody (ab45424) at 2 µg/ml + Daudi cell lysate at 15 µg

      Predicted band size: 18 kDa
      Observed band size: 27 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MD1 antibody (ab45424)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MD1 antibody (ab45424)

      ab45424 at 2.5µg/ml staining MD1 in human spleen tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

    References (1)

    Publishing research using ab45424? Please let us know so that we can cite the reference in this datasheet.

    ab45424 has been referenced in 1 publication.

    • Song Z  et al. Comprehensive Proteomic Profiling of Urinary Exosomes and Identification of Potential Non-invasive Early Biomarkers of Alzheimer's Disease in 5XFAD Mouse Model. Front Genet 11:565479 (2020). PubMed: 33250918

    Customer reviews and Q&As

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    Question

    Inquiry: Hi, I am interested in an antibody against MD-1, specifically used for immunohistochemistry and western blots in rat neuronal tissue. An advice you can give on the best antibody available would be great. Thanks.

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    Answer

    Thank you for contacting us.

    We have 8 MD1 antibodies in our catalogue: https://www.abcam.com/index.html?t=73113&pt=1.
    The anti-MD1 guaranteed to work in Western Blot, IHC-Paraffin and on Rat samples is ab45424, https://www.abcam.com/ab45424.

    Abpromise guarantee: we are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    AbTrial offer: if a product is to be used in an untested species or application, you may be eligible for our AbTrial program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Or you can find more information about this program at the following link: https://www.abcam.com/abtrial.

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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