Anti-MD1 antibody (ab45424)
Key features and details
- Rabbit polyclonal to MD1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MD1 antibody
See all MD1 primary antibodies -
Description
Rabbit polyclonal to MD1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic 15 amino acid peptide from near the center of human MD1
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Positive control
- Daudi cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody is an immunoaffinity purified IgG -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 2.5 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 18 kDa).
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Notes |
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IHC-P
Use a concentration of 2.5 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 18 kDa). |
Target
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Function
May cooperate with CD180 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) and cytokine production. Important for efficient CD180 cell surface expression. -
Tissue specificity
Highly expressed in B-cells, monocytes and tonsil. -
Cellular localization
Secreted > extracellular space. Associated with CD180 at the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 9450 Human
- Omim: 605241 Human
- SwissProt: O95711 Human
- Unigene: 653138 Human
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Alternative names
- BPAD antibody
- dJ80N2.1 antibody
- Ly 86 antibody
see all
Images
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Anti-MD1 antibody (ab45424) at 2 µg/ml + Daudi cell lysate at 15 µg
Predicted band size: 18 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted? -
ab45424 at 2.5µg/ml staining MD1 in human spleen tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab45424 has been referenced in 2 publications.
- Song Z et al. Comprehensive Proteomic Profiling of Urinary Exosomes and Identification of Potential Non-invasive Early Biomarkers of Alzheimer's Disease in 5XFAD Mouse Model. Front Genet 11:565479 (2020). PubMed: 33250918
- Yang HJ et al. Knockout of MD1 contributes to sympathetic hyperactivity and exacerbates ventricular arrhythmias following heart failure with preserved ejection fraction via NLRP3 inflammasome activation. Exp Physiol 105:966-978 (2020). PubMed: 32240565