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Our Abpromise guarantee covers the use of ab24182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 18 kDa).Can be blocked with Human MD2 peptide (ab39815).|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
ab24182 at 2µg/ml staining of MD2 in rat spleen cells by IHC
ab 24182 at a 1/100 dilution staining Rat mixed primary glial cell culture by FACS analysis. The antibody was incubated with the cells for 45 minutes and then detected using an Alexa-Fluor ® 488 Goat anti-rabbit antibody. For the negative control, primary antibody was omitted.
This image is courtesy of an Abreview by Nancy Nutile-McMenemy submitted on 16 March 2006.
ab24182 at a 1/200 dilution staining rat spinal cord tissue sections from a 4% PFA transcardially perfused animal by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and incubated with the antibody for 18 hours. Bound antibody was detected using an HRP conjugated goat anti-mouse polyclonal antibody.
This image is courtesy of an Abreview submitted by Nancy Nutile-McMenemy.
Immunohistochemical analysis of human spleen tissue stained for MD2 using ab24182 at 2.5μg/mL.
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