Product nameAnti-MD2 antibody
See all MD2 primary antibodies
DescriptionRabbit polyclonal to MD2
Tested applicationsSuitable for: Flow Cyt, IHC-Fr, WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: Mouse spleen lysate. ICC/IF: Rat and human spleen cells. IHC-P: Rat and human spleen tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
Purification notesVia peptide column.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab24182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 18 kDa).Can be blocked with Human MD2 peptide (ab39815).|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
FunctionCooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both MD2 and TLR4, but not TLR4 alone, respond to LPS.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- ESOP 1 antibody
- ESOP-1 antibody
- ESOP1 antibody
Anti-MD2 antibody (ab24182) at 1 µg/ml (mouse spleen lysate)
Predicted band size: 18 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
All lanes : Anti-MD2 antibody (ab24182) at 2 µg/ml (in 5% milk in TBS/T 0.1% (blocking buffer) for 12 hours at 4°C)
Lane 1 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours
Lane 2 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and LPS (50 ng/ml) for 10 minutes
Lane 3 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and ethanol (50 mM) for 10 minutes
Lane 4 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours
Lane 5 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and LPS (50 ng/ml) for 10 minutes
Lane 6 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and ethanol (50 mM) for 10 minutes
Lysates/proteins at 40 µg per lane.
All lanes : An HRP-conjugated Goat anti-rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
Blocking Step: 5% Milk for 1 hour at room temperature.
ab24182 at 2µg/ml staining of MD2 in rat spleen cells by IHC
ab 24182 at a 1/100 dilution staining Rat mixed primary glial cell culture by FACS analysis. The antibody was incubated with the cells for 45 minutes and then detected using an Alexa-Fluor ® 488 Goat anti-rabbit antibody. For the negative control, primary antibody was omitted.
This image is courtesy of an Abreview by Nancy Nutile-McMenemy submitted on 16 March 2006.
ab24182 at a 1/200 dilution staining rat spinal cord tissue sections from a 4% PFA transcardially perfused animal by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and incubated with the antibody for 18 hours. Bound antibody was detected using an HRP conjugated goat anti-mouse polyclonal antibody.
This image is courtesy of an Abreview submitted by Nancy Nutile-McMenemy.
Flow cytometry analysis of Human corneal epithelial cell lines, HCEC and HCET, staining MD2 with ab24182.
Cells were either unstimulated (unstim - black line) or stimulated (+IFN-γ - grey histogram), fixed in paraformaldehyde and incubated with primary antibody for 45 minutes at 4°C. A fluorescent-conjugated secondary antibody was used to detect staining.
Immunohistochemical analysis of human spleen tissue stained for MD2 using ab24182 at 2.5μg/mL.
This product has been referenced in:
- Guo J et al. LPS/TLR4-mediated stromal cells acquire an invasive phenotype and are implicated in the pathogenesis of adenomyosis. Sci Rep 6:21416 (2016). IHC-P ; Human . Read more (PubMed: 26898650) »
- Zou Y et al. sTLR4/MD-2 complex inhibits colorectal cancer in vitro and in vivo by targeting LPS. Oncotarget 7:52032-52044 (2016). IHC . Read more (PubMed: 27409669) »