Key features and details
- Rabbit polyclonal to MDA5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MDA5 antibody
See all MDA5 primary antibodies
DescriptionRabbit polyclonal to MDA5
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
16 amino acid peptide from near the carboxy terminus of human MDA5.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab79055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 117 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionRNA helicase that, through its ATP-dependent unwinding of RNA, may function to promote message degradation by specific RNases. Seems to have growth suppressive properties. Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). ATPase activity is specifically induced by dsRNA. Essential for the production of interferons in response to picornaviruses.
Tissue specificityWidely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung.
Involvement in diseaseGenetic variation in IFIH1 is associated with diabetes mellitus insulin-dependent type 19 (IDDM19) [MIM:610155]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Note=IFIH1 is the CADM-140 autoantigen, involved in clinically amyopathic dermatomyositis (CADM). This is a chronic inflammatory disorder that shows typical skin manifestations of dermatomyositis but has no or little evidence of clinical myositis. Anti-CADM-140 antibodies appear to be specific to dermatomyositis, especially CADM. Patients with anti-CADM-140 antibodies frequently develop life-threatening acute progressive interstitial lung disease (ILD).
Sequence similaritiesBelongs to the helicase family.
Contains 2 CARD domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
modificationsDuring apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation.
Cellular localizationCytoplasm. Nucleus. May be found in the nucleus, during apoptosis.
- Information by UniProt
- CADM-140 autoantigen antibody
- Clinically amyopathic dermatomyositis autoantigen 140 kDa antibody
- DEAD/H (Asp Glu Ala Asp/His) box polypeptide antibody
Immunohistochemical analysis of MDA5 expression in paraffin embedded, formalin fixed mouse kidney using 5 µg/ml ab79055
Lane 1 : Anti-MDA5 antibody (ab79055) at 1 µg/ml
Lane 2 : Anti-MDA5 antibody (ab79055) at 2 µg/ml
Lane 3 : Anti-MDA5 antibody (ab79055) at 4 µg/ml
All lanes : Daudi cell lysate
Predicted band size: 117 kDa
ICC/IF image of ab79055 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79055, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of MDA5 expression in paraffin embedded, formalin fixed mouse pancreas using 5 µg/ml ab79055
Immunohistochemical analysis of MDA5 expression in paraffin embedded, formalin fixed mouse small intestine using 5 µg/ml ab79055
Immunohistochemical analysis of MDA5 expression in paraffin embedded, formalin fixed human pancreas using 5 µg/ml ab79055
ab79055 has been referenced in 5 publications.
- Wang SQ et al. Knockdown of IGF-1R Triggers Viral RNA Sensor MDA5- and RIG-I-Mediated Mitochondrial Apoptosis in Colonic Cancer Cells. Mol Ther Nucleic Acids 16:105-117 (2019). PubMed: 30861413
- Kumthip K et al. Pivotal role for the ESCRT-II complex subunit EAP30/SNF8 in IRF3-dependent innate antiviral defense. PLoS Pathog 13:e1006713 (2017). WB . PubMed: 29084253
- Duewell P et al. Targeted activation of melanoma differentiation-associated protein 5 (MDA5) for immunotherapy of pancreatic carcinoma. Oncoimmunology 4:e1029698 (2015). IHC . PubMed: 26504669
- de Rivero Vaccari JP et al. Astrogliosis involves activation of retinoic acid-inducible gene-like signaling in the innate immune response after spinal cord injury. Glia 60:414-21 (2012). WB . PubMed: 22161971
- Berger M et al. Neutrophils express distinct RNA receptors in a non-canonical way. J Biol Chem 287:19409-17 (2012). WB, ICC/IF, Flow Cyt ; Human . PubMed: 22532562