Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-MDA5 antibody [EPR6743] (ab126630)

Knockout Tested Rabbit recombinant monoclonal MDA5 antibody [EPR6743]. Validated in WB, ICC/IF and tested in Human. Cited in 1 publication(s). Independently reviewed in 2 review(s).

Overview

  • Product name

    Anti-MDA5 antibody [EPR6743]
    See all MDA5 primary antibodies
  • Description

    Rabbit monoclonal [EPR6743] to MDA5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human MDA5. (Q9BYX4)

  • Positive control

    • THP1 treated with LPS cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 117 kDa.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      RNA helicase that, through its ATP-dependent unwinding of RNA, may function to promote message degradation by specific RNases. Seems to have growth suppressive properties. Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). ATPase activity is specifically induced by dsRNA. Essential for the production of interferons in response to picornaviruses.
    • Tissue specificity

      Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung.
    • Involvement in disease

      Genetic variation in IFIH1 is associated with diabetes mellitus insulin-dependent type 19 (IDDM19) [MIM:610155]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
      Note=IFIH1 is the CADM-140 autoantigen, involved in clinically amyopathic dermatomyositis (CADM). This is a chronic inflammatory disorder that shows typical skin manifestations of dermatomyositis but has no or little evidence of clinical myositis. Anti-CADM-140 antibodies appear to be specific to dermatomyositis, especially CADM. Patients with anti-CADM-140 antibodies frequently develop life-threatening acute progressive interstitial lung disease (ILD).
    • Sequence similarities

      Belongs to the helicase family.
      Contains 2 CARD domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Post-translational
      modifications

      During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation.
    • Cellular localization

      Cytoplasm. Nucleus. May be found in the nucleus, during apoptosis.
    • Information by UniProt
    • Database links

    • Alternative names

      • CADM-140 autoantigen antibody
      • Clinically amyopathic dermatomyositis autoantigen 140 kDa antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide antibody
      • DEAD/H box polypeptide antibody
      • Helicard antibody
      • Helicase with 2 CARD domains antibody
      • Hlcd antibody
      • IDDM 19 antibody
      • IDDM19 antibody
      • IFIH 1 antibody
      • Ifih1 antibody
      • IFIH1_HUMAN antibody
      • Interferon induced helicase C domain containing protein 1 antibody
      • interferon induced with helicase C domain 1 antibody
      • Interferon induced with helicase C domain protein 1 antibody
      • Interferon-induced helicase C domain-containing protein 1 antibody
      • Interferon-induced with helicase C domain protein 1 antibody
      • MDA 5 antibody
      • MDA-5 antibody
      • Melanoma differentiation associated protein 5 antibody
      • Melanoma differentiation-associated gene 5 antibody
      • Melanoma differentiation-associated protein 5 antibody
      • MGC133047 antibody
      • Murabutide down regulated protein antibody
      • Murabutide down-regulated protein antibody
      • RH 116 antibody
      • RH116 antibody
      • RIG I like receptor 2 antibody
      • RLR 2 antibody
      • RNA helicase DEAD box protein 116 antibody
      • RNA helicase-DEAD box protein 116 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MDA5 knockout HAP1 cell lysate (20 µg)
      Lane 3: THP cell lysate with 100 nM PAM for 6 h (20 µg)
      Lane 4: THP cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab126630 observed at 130 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab126630 was shown to specifically react with MDA5 when MDA5 knockout samples were used. Wild-type and MDA5 knockout samples were subjected to SDS-PAGE. ab126630 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Anti-MDA5 antibody [EPR6743] (ab126630) at 1/1000 dilution + THP1 treated with LPS cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 117 kDa

    References

    This product has been referenced in:

    • Mateer EJ  et al. Visualization of Double-Stranded RNA Colocalizing With Pattern Recognition Receptors in Arenavirus Infected Cells. Front Cell Infect Microbiol 8:251 (2018). Read more (PubMed: 30087859) »
    • Zhang Y  et al. Hantaan virus infection induces CXCL10 expression through TLR3, RIG-I, and MDA-5 pathways correlated with the disease severity. Mediators Inflamm 2014:697837 (2014). WB ; Human . Read more (PubMed: 24701034) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK-293T (human embryonic kidney))
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    10 µg
    Treatment
    24hrs 1000u/ml IFN-alpha
    Specification
    HEK-293T (human embryonic kidney)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Mr. Sam Hardy

    Verified customer

    Submitted Dec 14 2017

    Application
    Western blot
    Sample
    Pig Cell lysate - whole cell (PK-15 (porcine kidney, epithelial))
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    10 µg
    Treatment
    24hrs 1000u/ml IFN-alpha
    Specification
    PK-15 (porcine kidney, epithelial)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Mr. Sam Hardy

    Verified customer

    Submitted Dec 14 2017

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