Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-MDA5 antibody [EPR6743] - BSA and Azide free (ab219952)

Overview

  • Product name

    Anti-MDA5 antibody [EPR6743] - BSA and Azide free
    See all MDA5 primary antibodies
  • Description

    Rabbit monoclonal [EPR6743] to MDA5 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MDA5 aa 950-1050. The exact sequence is proprietary.
    Database link: Q9BYX4

  • General notes

    Ab219952 is the carrier-free version of ab126630. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab219952 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab219952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 117 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      RNA helicase that, through its ATP-dependent unwinding of RNA, may function to promote message degradation by specific RNases. Seems to have growth suppressive properties. Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). ATPase activity is specifically induced by dsRNA. Essential for the production of interferons in response to picornaviruses.
    • Tissue specificity

      Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung.
    • Involvement in disease

      Genetic variation in IFIH1 is associated with diabetes mellitus insulin-dependent type 19 (IDDM19) [MIM:610155]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
      Note=IFIH1 is the CADM-140 autoantigen, involved in clinically amyopathic dermatomyositis (CADM). This is a chronic inflammatory disorder that shows typical skin manifestations of dermatomyositis but has no or little evidence of clinical myositis. Anti-CADM-140 antibodies appear to be specific to dermatomyositis, especially CADM. Patients with anti-CADM-140 antibodies frequently develop life-threatening acute progressive interstitial lung disease (ILD).
    • Sequence similarities

      Belongs to the helicase family.
      Contains 2 CARD domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Post-translational
      modifications

      During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation.
    • Cellular localization

      Cytoplasm. Nucleus. May be found in the nucleus, during apoptosis.
    • Information by UniProt
    • Database links

    • Alternative names

      • CADM-140 autoantigen antibody
      • Clinically amyopathic dermatomyositis autoantigen 140 kDa antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide antibody
      • DEAD/H box polypeptide antibody
      • Helicard antibody
      • Helicase with 2 CARD domains antibody
      • Hlcd antibody
      • IDDM 19 antibody
      • IDDM19 antibody
      • IFIH 1 antibody
      • Ifih1 antibody
      • IFIH1_HUMAN antibody
      • Interferon induced helicase C domain containing protein 1 antibody
      • interferon induced with helicase C domain 1 antibody
      • Interferon induced with helicase C domain protein 1 antibody
      • Interferon-induced helicase C domain-containing protein 1 antibody
      • Interferon-induced with helicase C domain protein 1 antibody
      • MDA 5 antibody
      • MDA-5 antibody
      • Melanoma differentiation associated protein 5 antibody
      • Melanoma differentiation-associated gene 5 antibody
      • Melanoma differentiation-associated protein 5 antibody
      • MGC133047 antibody
      • Murabutide down regulated protein antibody
      • Murabutide down-regulated protein antibody
      • RH 116 antibody
      • RH116 antibody
      • RIG I like receptor 2 antibody
      • RLR 2 antibody
      • RNA helicase DEAD box protein 116 antibody
      • RNA helicase-DEAD box protein 116 antibody
      see all

    References

    ab219952 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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