Anti-MDC1 antibody (ab11170)
Key features and details
- Rabbit polyclonal to MDC1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MDC1 antibody
See all MDC1 primary antibodies -
Description
Rabbit polyclonal to MDC1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within MDC1 aa 900-1000. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q14676 -
Positive control
- WB: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
WB |
1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa).
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa). |
Target
-
Function
Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. -
Tissue specificity
Highly expressed in testis. -
Sequence similarities
Contains 2 BRCT domains.
Contains 1 FHA domain. -
Domain
Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX. -
Post-translational
modificationsPhosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. -
Cellular localization
Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks. - Information by UniProt
-
Database links
- Entrez Gene: 9656 Human
- GenBank: NP_055456 Human
- Omim: 607593 Human
- SwissProt: Q14676 Human
- Unigene: 653495 Human
-
Alternative names
- Homologue to Drosophila photoreceptor protein calphotin antibody
- MDC 1 antibody
- Mdc1 antibody
see all
Images
-
Lane 1 : Anti-MDC1 antibody (ab11170) at 4 µg/ml
Lane 2 : Anti-MDC1 antibody (ab11170) at 2 µg/ml
Lane 3 : Anti-MDC1 antibody (ab11170) at 1 µg/ml
Lane 4 : Anti-MDC1 antibody (ab11170) at 0.5 µg/ml
All lanes : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 220 kDa
Exposure time: 5 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MDC1 with ab11170 at 1/1000 (1µg/ml). Detection: DAB.
-
IHC image of MDC1 staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11170, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab11170 has been referenced in 3 publications.
- Lee JH et al. MicroRNA-22 Suppresses DNA Repair and Promotes Genomic Instability through Targeting of MDC1. Cancer Res 75:1298-310 (2015). PubMed: 25627978
- Williamson LM & Lees-Miller SP Estrogen receptor {alpha}-mediated transcription induces cell cycle-dependent DNA double-strand breaks. Carcinogenesis 32:279-85 (2011). Human . PubMed: 21112959
- Bu Y et al. Sp1-mediated transcriptional regulation of NFBD1/MDC1 plays a critical role in DNA damage response pathway. Genes Cells 13:53-66 (2008). WB ; Human . PubMed: 18173747