Anti-MDC1 antibody (ab11171)
Key features and details
- Rabbit polyclonal to MDC1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MDC1 antibody
See all MDC1 primary antibodies -
Description
Rabbit polyclonal to MDC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Synthetic peptide corresponding to Human MDC1 aa 1375-1425.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/5000 - 1/25000. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa).
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IP |
Use at 2-4 µg/mg of lysate.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/5000 - 1/25000. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa). |
IP
Use at 2-4 µg/mg of lysate. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. -
Tissue specificity
Highly expressed in testis. -
Sequence similarities
Contains 2 BRCT domains.
Contains 1 FHA domain. -
Domain
Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX. -
Post-translational
modificationsPhosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. -
Cellular localization
Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks. - Information by UniProt
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Database links
- Entrez Gene: 9656 Human
- GenBank: NP_055456 Human
- Omim: 607593 Human
- SwissProt: Q14676 Human
- Unigene: 653495 Human
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Alternative names
- Homologue to Drosophila photoreceptor protein calphotin antibody
- MDC 1 antibody
- Mdc1 antibody
see all
Images
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All lanes : Anti-MDC1 antibody (ab11171) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 220 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MDC1 with ab11171 at 1/1000 (1µg/ml). Detection: DAB.
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1 mg of HeLa whole cell lysate was incubated with ab11171, 3 µg/mL. For western blotting ab11171, 1 µg/mL was used to confirm successful immunoprecipation.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (31)
ab11171 has been referenced in 31 publications.
- De Marco Zompit M et al. The CIP2A-TOPBP1 complex safeguards chromosomal stability during mitosis. Nat Commun 13:4143 (2022). PubMed: 35842428
- Lee KY & Dutta A Chk1 promotes non-homologous end joining in G1 through direct phosphorylation of ASF1A. Cell Rep 34:108680 (2021). PubMed: 33503415
- Mertens ME & Knipe DM Herpes Simplex Virus 1 Manipulates Host Cell Antiviral and Proviral DNA Damage Responses. mBio 12:N/A (2021). PubMed: 33563816
- Sottnik JL et al. Mediator of DNA Damage Checkpoint 1 (MDC1) Is a Novel Estrogen Receptor Coregulator in Invasive Lobular Carcinoma of the Breast. Mol Cancer Res 19:1270-1282 (2021). PubMed: 33947745
- Janzen NR et al. Mice with Whole-Body Disruption of AMPK-Glycogen Binding Have Increased Adiposity, Reduced Fat Oxidation and Altered Tissue Glycogen Dynamics. Int J Mol Sci 22:N/A (2021). PubMed: 34502525