Overview

  • Product name

    Anti-MDC1 antibody [MDC1-50]
    See all MDC1 primary antibodies
  • Description

    Mouse monoclonal [MDC1-50] to MDC1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, ICC, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Monkey
  • Immunogen

    Recombinant fragment, corresponding to amino acids 2-200 of Human MDC1

  • Positive control

    • WB: total cell extract of G361 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purification notes

    This antibody is a purified Mouse Immunoglobulin.
  • Clonality

    Monoclonal
  • Clone number

    MDC1-50
  • Myeloma

    NS1
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 226 kDa.
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • Function

    Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
  • Tissue specificity

    Highly expressed in testis.
  • Sequence similarities

    Contains 2 BRCT domains.
    Contains 1 FHA domain.
  • Domain

    Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
  • Post-translational
    modifications

    Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
  • Cellular localization

    Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homologue to Drosophila photoreceptor protein calphotin antibody
    • MDC 1 antibody
    • Mdc1 antibody
    • MDC1_HUMAN antibody
    • Mediation of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint protein 1 antibody
    • NFBD 1 antibody
    • NFBD1 antibody
    • Nuclear factor with BRCT domains 1 antibody
    • Nuclear Factor with BRCT Domains Protein 1 antibody
    see all

Images

  • ab50003 staining MDC1 in human U2OS cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, permeabilized using NP40, blocked with 3% BSA for 3 hours at 25°C and then incubated with ab50003 at a 1/200 dilution for 1 hour at 25°C. The secondary used was an Alexa-Fluor 488 conjugated sheep anti-mouse IgG polyclonal used at a 1/500 dilution.

    MDC1 appears as green foci, the nucleus has been stained with DAPI (blue).

    See Abreview

References

This product has been referenced in:

  • Lee KY  et al. ASF1a Promotes Non-homologous End Joining Repair by Facilitating Phosphorylation of MDC1 by ATM at Double-Strand Breaks. Mol Cell 68:61-75.e5 (2017). Read more (PubMed: 28943310) »
  • Pederiva C  et al. Splicing controls the ubiquitin response during DNA double-strand break repair. Cell Death Differ 23:1648-57 (2016). Read more (PubMed: 27315300) »
See all 8 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton 5 minutes
Specification
HeLa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 20 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (smooth muscle cells)
Specification
smooth muscle cells
Fixative
Acetone
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 26 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Specification
U2OS
Fixative
Formaldehyde
Permeabilization
Yes - NP40
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Dr. Andrew Cobb

Verified customer

Submitted Mar 13 2012

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