Overview

  • Product name

    Anti-MDC1 antibody [P2B11]
    See all MDC1 primary antibodies
  • Description

    Mouse monoclonal [P2B11] to MDC1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment (GST-tag) corresponding to Mouse MDC1. (From around the N-terminal of NP_001010833.2)
    Database link: Q5PSV9

  • Positive control

    • ICC/IF: NIH/3T3 cells. WB: Human 293Trap cell lysates. Mouse cortex and cerebellum lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    P2B11
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000.
ICC/IF 1/100.

Target

  • Function

    Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
  • Tissue specificity

    Highly expressed in testis.
  • Sequence similarities

    Contains 2 BRCT domains.
    Contains 1 FHA domain.
  • Domain

    Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
  • Post-translational
    modifications

    Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
  • Cellular localization

    Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homologue to Drosophila photoreceptor protein calphotin antibody
    • MDC 1 antibody
    • Mdc1 antibody
    • MDC1_HUMAN antibody
    • Mediation of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint protein 1 antibody
    • NFBD 1 antibody
    • NFBD1 antibody
    • Nuclear factor with BRCT domains 1 antibody
    • Nuclear Factor with BRCT Domains Protein 1 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling MDC1 (Green) using ab241048 at 1:100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.

    Secondary antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 minutes at RT.

    Counterstain: DAPI (blue) nuclear stain at 1:5000, 5 minutes RT.

    (A) DAPI (blue) nuclear stain (B) ab241048 (C) Composite.

  • All lanes : Anti-MDC1 antibody [P2B11] (ab241048) at 1/1000 dilution (2 hours at RT)

    Lane 1 : Mouse cortex lysate
    Lane 2 : Mouse cerebellum lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse HRP-IgG for 60 minutes at RT at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 5 minutes


    Block: 5% Skimmed milk in 1X TBST

  • Anti-MDC1 antibody [P2B11] (ab241048) at 1/1000 dilution (2 hours at RT) + Human 293Trap cell lysates at 30 µg

    Secondary
    Goat anti-Mouse HRP-IgG for 60 minutes at RT at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 5 minutes


     Block: 5% Skimmed milk in 1X TBST.

References

ab241048 has not yet been referenced specifically in any publications.

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