Overview

  • Product name

    Anti-MDC1 (phospho S964) antibody
    See all MDC1 primary antibodies
  • Description

    Rabbit polyclonal to MDC1 (phospho S964)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Non human primates
  • Immunogen

    Synthetic peptide corresponding to Human MDC1 aa 950-1050 (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab36491, ab36492)

  • Positive control

    • This antibody gave a positive signal in SW480 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab36017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
WB 1/250. Detects a band of approximately 270 kDa (predicted molecular weight: 226 kDa).

Target

  • Function

    Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
  • Tissue specificity

    Highly expressed in testis.
  • Sequence similarities

    Contains 2 BRCT domains.
    Contains 1 FHA domain.
  • Domain

    Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
  • Post-translational
    modifications

    Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
  • Cellular localization

    Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homologue to Drosophila photoreceptor protein calphotin antibody
    • MDC 1 antibody
    • Mdc1 antibody
    • MDC1_HUMAN antibody
    • Mediation of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint protein 1 antibody
    • NFBD 1 antibody
    • NFBD1 antibody
    • Nuclear factor with BRCT domains 1 antibody
    • Nuclear Factor with BRCT Domains Protein 1 antibody
    see all

Images

  • IHC image of MDC1 staining in Human Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36017, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab36017 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Specification
Skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
Yes - TBST
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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