Anti-MDC1 (phospho T4) antibody (ab35967)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-MDC1 (phospho T4) antibody
See all MDC1 primary antibodies -
DescriptionRabbit polyclonal to MDC1 (phospho T4)
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MDC1, phosphorylated at T4.
(Peptide available as ab36513.)
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Positive control
- This antibody gave a positive signal in the following Human Cell Lysates: HepG2 Nuclear - IFN gamma treated HeLa Whole Cell - UV Irradiated (data not shown)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab35967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 25934699 | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 287 kDa (predicted molecular weight: 227 kDa). |
Target
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FunctionRequired for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
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Tissue specificityHighly expressed in testis.
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Sequence similaritiesContains 2 BRCT domains.
Contains 1 FHA domain. -
DomainTandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
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Post-translational
modificationsPhosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. -
Cellular localizationNucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
- Information by UniProt
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Database links
- Entrez Gene: 9656 Human
- Entrez Gene: 240087 Mouse
- GenBank: NP_055456 Human
- Omim: 607593 Human
- SwissProt: Q14676 Human
- SwissProt: Q5PSV9 Mouse
- Unigene: 653495 Human
- Unigene: 218511 Mouse
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Alternative names
- Homologue to Drosophila photoreceptor protein calphotin antibody
- MDC 1 antibody
- Mdc1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MDC1 (phospho T4) antibody (ab35967)This image is courtesy of an anonymous Abreviewab35967 staining MDC1 (phospho T4) in HeLa cells (untreated or treated with 10 Gy IR) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour. Samples were incubated with primary antibody (1/200 in 5% BSA) for 1 hour. A FITC-conjugated donkey anti-rabbit IgG polyclonal (1/150) was used as the secondary antibody.
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ELISA - Anti-MDC1 (phospho T4) antibody (ab35967) -
Western blot - Anti-MDC1 (phospho T4) antibody (ab35967)
References
This product has been referenced in:
- Modzelewski AJ et al. Dgcr8 and Dicer are essential for sex chromosome integrity during meiosis in males. J Cell Sci 128:2314-27 (2015). IHC-P ; Mouse . Read more (PubMed: 25934699) »
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