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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MDH1 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: P40925
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab180152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling MDH1 with ab180152 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution. Counter stained with DAPI.
Western blot analysis of HepG2 cell lysate immunoprecipitated with ab180152 at 1/50 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling MDH1 with ab180152 at 1/20 dilution followed by Goat anti rabbit IgG (FITC) at 1/75 dilution (red), compared to a Rabbit IgG control (green).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"