Overview

  • Product name

  • Description

    Goat polyclonal to MDH2
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cow, Cat, Cynomolgus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human MDH2 aa 109-119. (NP_005909.2).
    Sequence:

    C-TRDDLFNTNAT


    Database link: P40926

  • Positive control

    • WB: HeLa, HepG2 and NIH/3T3 whole cell lysate; Human, mouse, rat and pig heart tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab240428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml.

Target

  • Sequence similarities

    Belongs to the LDH/MDH superfamily. MDH type 1 family.
  • Post-translational
    modifications

    Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri-and tetraacetylations. Glucose also increases acetylation by about 60%.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • M MDH antibody
    • Malate dehydrogenase 2, NAD (mitochondrial) antibody
    • Malate dehydrogenase antibody
    • Malate dehydrogenase, mitochondrial antibody
    • MDH antibody
    • mdh2 antibody
    • MDHM_HUMAN antibody
    • MGC:3559 antibody
    • mitochondrial antibody
    • Mitochondrial malate dehydrogenase 2, NAD antibody
    • Mor 1 antibody
    • MOR1 antibody
    see all

Images

  • All lanes : Anti-MDH2 antibody (ab240428) at 0.01 µg/ml

    Lane 1 : Human heart tissue lysate (in RIPA buffer)
    Lane 2 : Mouse heart tissue lysate (in RIPA buffer)
    Lane 3 : Rat heart tissue lysate (in RIPA buffer)
    Lane 4 : Pig heart tissue lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.
  • All lanes : Anti-MDH2 antibody (ab240428) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

References

ab240428 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab240428.
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