Overview

  • Product name

    Anti-MDH2 antibody [EPR14883(B)]
    See all MDH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14883(B)] to MDH2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MDH2 aa 200-300. The exact sequence is proprietary.
    Database link: P40926

  • Positive control

    • K562, HepG2, and HeLa whole cell lysate (ab150035); Human hepatocellular carcinoma tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/50.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Sequence similarities

    Belongs to the LDH/MDH superfamily. MDH type 1 family.
  • Post-translational
    modifications

    Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri-and tetraacetylations. Glucose also increases acetylation by about 60%.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • M MDH antibody
    • Malate dehydrogenase 2, NAD (mitochondrial) antibody
    • Malate dehydrogenase antibody
    • Malate dehydrogenase, mitochondrial antibody
    • MDH antibody
    • mdh2 antibody
    • MDHM_HUMAN antibody
    • MGC:3559 antibody
    • mitochondrial antibody
    • Mitochondrial malate dehydrogenase 2, NAD antibody
    • Mor 1 antibody
    • MOR1 antibody
    see all

Images

  • All lanes : Anti-MDH2 antibody [EPR14883(B)] (ab181857) at 1/50000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling MDH2 with ab181857 at 1/10 dilution (red) compared to a Rabbit IgG monoclonal isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling MDH2 with ab181857 at 1/50 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

References

This product has been referenced in:

  • Zhang P  et al. iTRAQ-based differential proteomic analysis of the brains in a rat model of delayedcarbon monoxide encephalopathy. Brain Res Bull 137:329-337 (2018). IHC-P . Read more (PubMed: 29355713) »
  • Zhuang Y  et al. MDH2 Stimulated by Estrogen-GPR30 Pathway Down-Regulated PTEN Expression Promoting the Proliferation and Invasion of Cells in Endometrial Cancer. Transl Oncol 10:203-210 (2017). Read more (PubMed: 28189066) »
See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181857.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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