Anti-MDM2 (phospho S166) antibody (ab131355)

Rabbit polyclonal MDM2 (phospho S166) antibody. Validated in WB, IHC, ICC/IF and tested in Human. Cited in 1 publication(s). Immunogen corresponding to synthetic peptide.

Overview

  • Product name

    Anti-MDM2 (phospho S166) antibody
    See all MDM2 primary antibodies
  • Description

    Rabbit polyclonal to MDM2 (phospho S166)
  • Host species

    Rabbit
  • Specificity

    ab131355 detects endogenous level of MDM2 only when phosphorylated at Serine 166.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, surrounding phosphorylation site of Serine 166 (

    A-I-S(p)-E-T

    ) derived from Human MDM2 (NP_002383.2).

  • Positive control

    • 293 cell extract treated with Hydroxyurea, HeLa cells, Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab131355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 55 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways.
  • Tissue specificity

    Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
  • Involvement in disease

    Note=Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.
  • Sequence similarities

    Belongs to the MDM2/MDM4 family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIB domain.
  • Domain

    Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the heterooligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself.
  • Post-translational
    modifications

    Phosphorylated in response to ionizing radiation in an ATM-dependent manner.
    Auto-ubiquitinated; which leads to proteasomal degradation. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitinilation and degradation of p53/TP53. Deubiquitinated by USP7; leading to stabilize it.
  • Cellular localization

    Nucleus > nucleoplasm. Cytoplasm. Nucleus > nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACTFS antibody
    • Double minute 2 protein antibody
    • E3 ubiquitin-protein ligase Mdm2 antibody
    • Hdm 2 antibody
    • Hdm2 antibody
    • HDMX antibody
    • MDM 2 antibody
    • MDM2 antibody
    • MDM2 oncogene E3 ubiquitin protein ligase antibody
    • Mdm2 p53 E3 ubiquitin protein ligase homolog antibody
    • Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody
    • MDM2_HUMAN antibody
    • MDM2BP antibody
    • Mouse Double Minute 2 antibody
    • MTBP antibody
    • Murine Double Minute Chromosome 2 antibody
    • Oncoprotein Mdm2 antibody
    • p53 Binding Protein Mdm2 antibody
    • p53-binding protein Mdm2 antibody
    • Ubiquitin protein ligase E3 Mdm2 antibody
    • Ubiquitin protein ligase E3 Mdm2 antibody
    see all

Images

  • All lanes : Anti-MDM2 (phospho S166) antibody (ab131355) at 1/500 dilution

    Lane 1 : 293 cell extract untreated
    Lane 2 : 293 cell extract treated with hydroxyurea

    Predicted band size: 55 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MDM2 (phospho S166) with ab131355 at a dilution of 1/50. Picture on the right was preincubated with blocking peptide.
  • Immunofluorescence of methanol-fixed HeLa cells staining MDM2 (phospho S166) with ab131355 at a dilution of 1/100.

References

This product has been referenced in:

  • Wu DM  et al. MircoRNA-1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1. J Cell Mol Med 22:4963-4974 (2018). Read more (PubMed: 30024092) »
  • Lim EG  et al. Ethanol extract from Cnidium monnieri (L.) Cusson induces cell cycle arrest and apoptosis via regulation of the p53-independent pathway in HepG2 and Hep3B hepatocellular carcinoma cells. Mol Med Rep 17:2572-2580 (2018). Read more (PubMed: 29207130) »
See all 2 Publications for this product

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