Product nameAnti-MDMX antibody [MDMX-82]
See all MDMX primary antibodies
DescriptionMouse monoclonal [MDMX-82] to MDMX
Tested applicationsSuitable for: ELISA, IP, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Xenopus laevis, Chimpanzee, Opossum
- Recombinant Human MDMX protein (ab114467) can be used as a positive control in WB.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
Purification notesPurified Immunoglobulin
Our Abpromise guarantee covers the use of ab49993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.|
FunctionInhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
Tissue specificityExpressed in all tissues tested with high levels in thymus.
Sequence similaritiesBelongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain.
DomainRegion I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
modificationsUbiquitinated. Deubiquitinated by USP2; leading to stabilize it.
- Information by UniProt
- DKFZp781B1423 antibody
- Double minute 4 antibody
- Double minute 4 human homolog of p53 binding protein antibody
Anti-MDMX antibody [MDMX-82] (ab49993) at 1/1000 dilution + Mouse myoblast cell line C2C12 whole cell lysate at 15 µg
HRP-conjugated sheep anti-mouse IgG polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
This product has been referenced in:
- Endo S et al. Potent in vitro and in vivo antitumor effects of MDM2 inhibitor nutlin-3 in gastric cancer cells. Cancer Sci 102:605-13 (2011). WB ; Human . Read more (PubMed: 21205074) »
- Marine JC & Jochemsen AG Mdmx as an essential regulator of p53 activity. Biochem Biophys Res Commun 331:750-60 (2005). Read more (PubMed: 15865931) »