Key features and details
- Rabbit polyclonal to MDMX/MDM4
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MDMX/MDM4 antibody
See all MDMX/MDM4 primary antibodies
DescriptionRabbit polyclonal to MDMX/MDM4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
- WB: Mouse stomach tissue lysate; HepG2 and HeLa whole cell lysate.
This product was previously labelled as MDMX
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab222905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
FunctionInhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
Tissue specificityExpressed in all tissues tested with high levels in thymus.
Sequence similaritiesBelongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain.
DomainRegion I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
modificationsUbiquitinated. Deubiquitinated by USP2; leading to stabilize it.
- Information by UniProt
- DKFZp781B1423 antibody
- Double minute 4 antibody
- Double minute 4 human homolog of p53 binding protein antibody
All lanes : Anti-MDMX/MDM4 antibody (ab222905) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : Mouse stomach tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/500000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
ab222905 has not yet been referenced specifically in any publications.