Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-3-2F2] to MDMX/MDM4
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-MDMX/MDM4 antibody [BL-3-2F2]
See all MDMX/MDM4 primary antibodies
DescriptionRabbit monoclonal [BL-3-2F2] to MDMX/MDM4
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human MDMX/MDM4 aa 125-175. The exact sequence is proprietary. NP_002384.2 and Gene ID 4194.
Database link: O15151
- WB: Jurkat, 293T, HeLa and A549 cell lysates. IP: Jurkat cell lysate.
This product is sold under License from Bethyl Laboratories, Inc.
This product was previously labelled as MDMX
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
PurityTissue culture supernatant
Purification notesCell culture supernatant was harvested from mammalian cells expressing recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab243859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 20µg/mg lysate.
FunctionInhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
Tissue specificityExpressed in all tissues tested with high levels in thymus.
Sequence similaritiesBelongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain.
DomainRegion I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
modificationsUbiquitinated. Deubiquitinated by USP2; leading to stabilize it.
- Information by UniProt
- DKFZp781B1423 antibody
- Double minute 4 antibody
- Double minute 4 human homolog of p53 binding protein antibody
MDMX/MDM4 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) using ab243859 at 20µg/mL. Western blot was performed from the immunoprecipitate using ab243859 at 1/100 dilution.
Lane 1: ab243859 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP.
Chemiluminescence with an exposure time of 75 seconds.
All lanes : Anti-MDMX/MDM4 antibody [BL-3-2F2] (ab243859)
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243859 has not yet been referenced specifically in any publications.