Product nameAnti-MDMX/MDM4 antibody [BL-3-2F2]
See all MDMX/MDM4 primary antibodies
DescriptionRabbit monoclonal [BL-3-2F2] to MDMX/MDM4
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human MDMX/MDM4 aa 125-175. The exact sequence is proprietary. NP_002384.2 and Gene ID 4194.
Database link: O15151
- WB: Jurkat, 293T, HeLa and A549 cell lysates. IP: Jurkat cell lysate.
This product is sold under License from Bethyl Laboratories, Inc.
This product was previously labelled as MDMX
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
PurityTissue culture supernatant
Purification notesCell culture supernatant was harvested from mammalian cells expressing recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab243859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 20µg/mg lysate.
FunctionInhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
Tissue specificityExpressed in all tissues tested with high levels in thymus.
Sequence similaritiesBelongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain.
DomainRegion I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
modificationsUbiquitinated. Deubiquitinated by USP2; leading to stabilize it.
- Information by UniProt
- DKFZp781B1423 antibody
- Double minute 4 antibody
- Double minute 4 human homolog of p53 binding protein antibody
MDMX/MDM4 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) using ab243859 at 20µg/mL. Western blot was performed from the immunoprecipitate using ab243859 at 1/100 dilution.
Lane 1: ab243859 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP.
Chemiluminescence with an exposure time of 75 seconds.
All lanes : Anti-MDMX/MDM4 antibody [BL-3-2F2] (ab243859)
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
ab243859 has not yet been referenced specifically in any publications.