Recombinant

Recombinant Anti-MDMX/MDM4 antibody [BL-3-2F2] (ab243859)

Overview

  • Product name

    Anti-MDMX/MDM4 antibody [BL-3-2F2]
    See all MDMX/MDM4 primary antibodies
  • Description

    Rabbit monoclonal [BL-3-2F2] to MDMX/MDM4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MDMX/MDM4 aa 125-175. The exact sequence is proprietary. NP_002384.2 and Gene ID 4194.
    Database link: O15151

  • Positive control

    • WB: Jurkat, 293T, HeLa and A549 cell lysates. IP: Jurkat cell lysate.
  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

     This product was previously labelled as MDMX

     

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab243859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IP Use at an assay dependent concentration.

Use 20µg/mg lysate.

Target

  • Function

    Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
  • Tissue specificity

    Expressed in all tissues tested with high levels in thymus.
  • Sequence similarities

    Belongs to the MDM2/MDM4 family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIB domain.
  • Domain

    Region I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
  • Post-translational
    modifications

    Ubiquitinated. Deubiquitinated by USP2; leading to stabilize it.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp781B1423 antibody
    • Double minute 4 antibody
    • Double minute 4 human homolog of p53 binding protein antibody
    • Double minute 4 protein antibody
    • HDMX antibody
    • MDM 4 antibody
    • Mdm2 like p53 binding protein antibody
    • Mdm2-like p53-binding protein antibody
    • MDM4 antibody
    • Mdm4 p53 binding protein homolog mouse antibody
    • Mdm4 protein antibody
    • MDM4 related protein 1 antibody
    • Mdm4 transformed 3T3 cell double minute 4 antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein mouse antibody
    • MDM4_HUMAN antibody
    • Mdmx protein antibody
    • MGC132766 antibody
    • Mouse double minute 4 homolog antibody
    • Mouse double minute 4 human homolog of p53 binding protein antibody
    • MRP 1 antibody
    • MRP1 antibody
    • p53 binding protein antibody
    • p53 BINDING PROTEIN MDM4 antibody
    • p53-binding protein Mdm4 antibody
    • Protein Mdm4 antibody
    • Protein Mdmx antibody
    see all

Images

  • MDMX/MDM4 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) using ab243859 at 20µg/mL. Western blot was performed from the immunoprecipitate using ab243859 at 1/100 dilution.

    Lane 1: ab243859 IP in Jurkat whole cell lysate.
    Lane 2: Control IgG IP.

    Chemiluminescence with an exposure time of 75 seconds.

  • All lanes : Anti-MDMX/MDM4 antibody [BL-3-2F2] (ab243859)

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : A549 cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

References

ab243859 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab243859.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up