Key features and details
- Rabbit polyclonal to MDMX/MDM4 (phospho S403)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MDMX/MDM4 (phospho S403) antibody
See all MDMX/MDM4 primary antibodies
DescriptionRabbit polyclonal to MDMX/MDM4 (phospho S403)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to Human MDMX/MDM4.
Database link: O15151
This product was previously labelled as MDMX
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/3000. Detects a band of approximately 80 kDa.|
FunctionInhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
Tissue specificityExpressed in all tissues tested with high levels in thymus.
Sequence similaritiesBelongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain.
DomainRegion I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
modificationsUbiquitinated. Deubiquitinated by USP2; leading to stabilize it.
- Information by UniProt
- DKFZp781B1423 antibody
- Double minute 4 antibody
- Double minute 4 human homolog of p53 binding protein antibody
All lanes : Anti-MDMX/MDM4 (phospho S403) antibody (ab70236) at 0.13 µg/ml
Lane 1 : 293T whole cell lysate with HA tagged-MDMX/MDM4 S403A mutant and NCS (2hrs at 200 ng/ml)
Lane 2 : 293T whole cell lysate with HA tagged-MDMX/MDM4 S403A mutant and NCS (0.5hrs at 200 ng/ml)
Lane 3 : 293T whole cell lysate with HA tagged-MDMX/MDM4 S403A mutant
Lane 4 : 293T whole cell lysate with HA tagged-MDMX/MDM4 (wild-type) and NCS (2hrs at 200 ng/ml)
Lane 5 : 293T whole cell lysate with HA tagged-MDMX/MDM4 (wild-type) and NCS (0.5hrs at 200 ng/ml)
Lane 6 : 293T whole cell lysate with HA tagged-MDMX/MDM4 (wild-type)
Lane 7 : mock transfected 293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Observed band size: 80 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
ab70236 has not yet been referenced specifically in any publications.