Key features and details
- Rabbit polyclonal to ME2 - C-terminal
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ME2 antibody - C-terminal
See all ME2 primary antibodies
DescriptionRabbit polyclonal to ME2 - C-terminal
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Recombinant fragment within Human ME2 (C terminal). The exact sequence is proprietary.
Database link: P23368
- WB: THP-1 and HL-60 whole cell extracts. IP: THP-1 whole cell extract. IHC-P: Mouse muscle tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/100 - 1/500.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 65 kDa.|
Sequence similaritiesBelongs to the malic enzymes family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Malate dehydrogenase antibody
- Malic enzyme 2 antibody
- Malic enzyme 2 mitochondrial antibody
All lanes : Anti-ME2 antibody - C-terminal (ab229471) at 1/1000 dilution
Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell extract
Lane 2 : HL-60 (human romyelocytic leukemia cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 65 kDa
7.5% SDS-PAGE gel.
ME2 was immunoprecipitated from THP-1 (human monocytic leukemia cell line) whole cell extract with 5 µg ab229471. Western blot was performed from the immunoprecipitate using ab229471. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: THP-1 whole cell extract.
Lane 2: Control IgG IP in THP-1 whole cell extract.
Lane 3: ab229471 IP in THP-1 whole cell extract.
Paraffin-embedded mouse muscle tissue stained for ME2 using ab229471 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229471 has not yet been referenced specifically in any publications.