Product nameAnti-ME2 antibody - C-terminal
See all ME2 primary antibodies
DescriptionRabbit polyclonal to ME2 - C-terminal
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Recombinant fragment within Human ME2 (C terminal). The exact sequence is proprietary.
Database link: P23368
- WB: THP-1 and HL-60 whole cell extracts. IP: THP-1 whole cell extract. IHC-P: Mouse muscle tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/100 - 1/500.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 65 kDa.|
Sequence similaritiesBelongs to the malic enzymes family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Malate dehydrogenase antibody
- Malic enzyme 2 antibody
- Malic enzyme 2 mitochondrial antibody
All lanes : Anti-ME2 antibody - C-terminal (ab229471) at 1/1000 dilution
Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell extract
Lane 2 : HL-60 (human romyelocytic leukemia cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 65 kDa
7.5% SDS-PAGE gel.
ME2 was immunoprecipitated from THP-1 (human monocytic leukemia cell line) whole cell extract with 5 µg ab229471. Western blot was performed from the immunoprecipitate using ab229471. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: THP-1 whole cell extract.
Lane 2: Control IgG IP in THP-1 whole cell extract.
Lane 3: ab229471 IP in THP-1 whole cell extract.
Paraffin-embedded mouse muscle tissue stained for ME2 using ab229471 at 1/500 dilution in immunohistochemical analysis.
ab229471 has not yet been referenced specifically in any publications.