Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7218] to ME2
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Product nameAnti-ME2 antibody [EPR7218]
See all ME2 primary antibodies
DescriptionRabbit monoclonal [EPR7218] to ME2
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Does not react with: Rat
Synthetic peptide, corresponding to residues in Human ME2. (Uniprot: P23368).
- Jurkat, HeLa, K562 and MOLT4 cell lysates; Human colon tissue; HeLa cells; 293T .
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab126616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).|
|IHC-P||1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/50 - 1/100.
|ICC/IF||1/100 - 1/250.|
Sequence similaritiesBelongs to the malic enzymes family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Malate dehydrogenase antibody
- Malic enzyme 2 antibody
- Malic enzyme 2 mitochondrial antibody
All lanes : Anti-ME2 antibody [EPR7218] (ab126616) at 1/5000 dilution (purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Blocking and diluting buffer: 5% NFDM/TBST
Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling ME2 with purified ab126616 at 1:150 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilized with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ME2 with purified ab126616 at 1:150 dilution (9.1μg/ml). Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling Me2 with Purified ab126616 at 1:1000 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
All lanes : Anti-ME2 antibody [EPR7218] (ab126616) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : K562 cell lysate
Lane 5 : MOLT4 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 65 kDa
Unpurified ab126616, at 1/50 dilution, staining ME2 in Formalin-fixed, Paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Unpurified ab126616, at 1/100 dilution, staining ME2 in HeLa cells by Immunofluorescence.
ab126616 has not yet been referenced specifically in any publications.