Recombinant
RabMAb

Recombinant Anti-ME2 antibody [EPR7218] (ab126616)

Overview

  • Product name

    Anti-ME2 antibody [EPR7218]
    See all ME2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7218] to ME2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Rat
  • Immunogen

    Synthetic peptide, corresponding to residues in Human ME2. (Uniprot: P23368).

  • Positive control

    • Jurkat, HeLa, K562 and MOLT4 cell lysates; Human colon tissue; HeLa cells; 293T .
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/50 - 1/100.

Flow Cyt 1/150.
ICC/IF 1/100 - 1/250.

Target

  • Sequence similarities

    Belongs to the malic enzymes family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Malate dehydrogenase antibody
    • Malic enzyme 2 antibody
    • Malic enzyme 2 mitochondrial antibody
    • Malic enzyme 2 NAD(+) dependent mitochondrial antibody
    • Malic enzyme mitochondrial antibody
    • Malic enzyme NAD(+) dependent mitochondrial antibody
    • MAOM_HUMAN antibody
    • ME 2 antibody
    • ME2 antibody
    • mitochondrial antibody
    • NAD dependent malic enzyme mitochondrial antibody
    • NAD ME antibody
    • NAD-dependent malic enzyme antibody
    • NAD-ME antibody
    • ODS1 antibody
    • Pyruvic malic carboxylase antibody
    see all

Images

  • All lanes : Anti-ME2 antibody [EPR7218] (ab126616) at 1/5000 dilution (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 65 kDa



    Blocking and diluting buffer: 5% NFDM/TBST 

  • Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling ME2 with purified ab126616 at 1:150 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilized with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ME2 with purified ab126616 at 1:150 dilution (9.1μg/ml). Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling Me2 with Purified ab126616 at 1:1000 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • All lanes : Anti-ME2 antibody [EPR7218] (ab126616) at 1/1000 dilution (unpurified)

    Lane 1 : 293T cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : MOLT4 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 65 kDa

  • Unpurified ab126616, at 1/50 dilution, staining ME2 in Formalin-fixed, Paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Unpurified ab126616, at 1/100 dilution, staining ME2 in HeLa cells by Immunofluorescence.

References

ab126616 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

For HeLa cells only, we fix with acetone at -20 ºC for 5 mintues and continue to washing and staining.

If you are using other cell types, we recommend fixing with 2% PFA for 20 min and permeabilizing with 0.1% Triton-X for 5 min.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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