RecombinantRabMAb

Recombinant Anti-ME2 antibody [EPR7218] (ab126616)

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Western blot - Anti-ME2 antibody [EPR7218] (ab126616)
  • Flow Cytometry - Anti-ME2 antibody [EPR7218] (ab126616)
  • Immunocytochemistry/ Immunofluorescence - Anti-ME2 antibody [EPR7218] (ab126616)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EPR7218] (ab126616)
  • Western blot - Anti-ME2 antibody [EPR7218] (ab126616)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EPR7218] (ab126616)
  • Immunocytochemistry/ Immunofluorescence - Anti-ME2 antibody [EPR7218] (ab126616)
  • Anti-ME2 antibody [EPR7218] (ab126616)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7218] to ME2
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-ME2 antibody [EPR7218]
    See all ME2 primary antibodies

  • Description

    Rabbit monoclonal [EPR7218] to ME2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Rat

  • Immunogen

    Synthetic peptide, corresponding to residues in Human ME2. (Uniprot: P23368).

  • Positive control

    • Jurkat, HeLa, K562 and MOLT4 cell lysates; Human colon tissue; HeLa cells; 293T .

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab126616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/50 - 1/100.
Flow Cyt 1/150.
ICC/IF 1/100 - 1/250.

Target

  • Sequence similarities

    Belongs to the malic enzymes family.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    • Alternative names

      • Malate dehydrogenase antibody
      • Malic enzyme 2 antibody
      • Malic enzyme 2 mitochondrial antibody
      • Malic enzyme 2 NAD(+) dependent mitochondrial antibody
      • Malic enzyme mitochondrial antibody
      • Malic enzyme NAD(+) dependent mitochondrial antibody
      • MAOM_HUMAN antibody
      • ME 2 antibody
      • ME2 antibody
      • mitochondrial antibody
      • NAD dependent malic enzyme mitochondrial antibody
      • NAD ME antibody
      • NAD-dependent malic enzyme antibody
      • NAD-ME antibody
      • ODS1 antibody
      • Pyruvic malic carboxylase antibody
      see all

    Images

    • Western blot - Anti-ME2 antibody [EPR7218] (ab126616)
      Western blot - Anti-ME2 antibody [EPR7218] (ab126616)
      All lanes : Anti-ME2 antibody [EPR7218] (ab126616) at 1/5000 dilution (purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 65 kDa



      Blocking and diluting buffer: 5% NFDM/TBST 

    • Flow Cytometry - Anti-ME2 antibody [EPR7218] (ab126616)
      Flow Cytometry - Anti-ME2 antibody [EPR7218] (ab126616)

      Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling ME2 with purified ab126616 at 1:150 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilized with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Immunocytochemistry/ Immunofluorescence - Anti-ME2 antibody [EPR7218] (ab126616)
      Immunocytochemistry/ Immunofluorescence - Anti-ME2 antibody [EPR7218] (ab126616)

      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ME2 with purified ab126616 at 1:150 dilution (9.1μg/ml). Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EPR7218] (ab126616)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EPR7218] (ab126616)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling Me2 with Purified ab126616 at 1:1000 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Western blot - Anti-ME2 antibody [EPR7218] (ab126616)
      Western blot - Anti-ME2 antibody [EPR7218] (ab126616)
      All lanes : Anti-ME2 antibody [EPR7218] (ab126616) at 1/1000 dilution (unpurified)

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : K562 cell lysate
      Lane 5 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 65 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EPR7218] (ab126616)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EPR7218] (ab126616)

      Unpurified ab126616, at 1/50 dilution, staining ME2 in Formalin-fixed, Paraffin-embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-ME2 antibody [EPR7218] (ab126616)
      Immunocytochemistry/ Immunofluorescence - Anti-ME2 antibody [EPR7218] (ab126616)

      Unpurified ab126616, at 1/100 dilution, staining ME2 in HeLa cells by Immunofluorescence.

    • Anti-ME2 antibody [EPR7218] (ab126616)
      Anti-ME2 antibody [EPR7218] (ab126616)

    References (0)

    Publishing research using ab126616? Please let us know so that we can cite the reference in this datasheet.

    ab126616 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question


    Inquiry: Hello, I wish to do immunofluorescence/ICC with the ME2 antibody EPR7218 and was wondering how the cells were fixed and permeabilized to give the immunofluorescence image of HeLa cells on the product website? As much detail as possible (i.e. fixative, concentration, incubation time, etc) would be greatly appreciated. Thank you.

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    Answer

    Thank you for contacting us.

    For HeLa cells only, we fix with acetone at -20 ºC for 5 mintues and continue to washing and staining.

    If you are using other cell types, we recommend fixing with 2% PFA for 20 min and permeabilizing with 0.1% Triton-X for 5 min.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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