RecombinantRabMAb

Recombinant Anti-ME3 antibody [EPR10378] (ab172972)

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Western blot - Anti-ME3 antibody [EPR10378] (ab172972)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (ab172972)
  • Western blot - Anti-ME3 antibody [EPR10378] (ab172972)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (ab172972)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (ab172972)
  • Anti-ME3 antibody [EPR10378] (ab172972)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10378] to ME3
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ME3 antibody [EPR10378]
    See all ME3 primary antibodies

  • Description

    Rabbit monoclonal [EPR10378] to ME3

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ME3 aa 550 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q16798

  • Positive control

    • Fetal brain, Fetal kidney, Fetal heart and HeLa lysates. paraffin-embedded Human brain tissue and Human thyroid tissue. Mouse heart, rat heart, rat brain and mouse brain lysates; human colon tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab172972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/50 - 1/100. 
WB 1/1000 - 1/10000. Predicted molecular weight: 67 kDa.

Target

  • Relevance

    Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+) dependent isoform, and a mitochondrial NAD(+) dependent isoform. This gene encodes a mitochondrial NADP(+) dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. ME3 is expressed predominantly in organs with a low division rate. Its catalytic activity is (S) malate + NADP+ = pyruvate + CO2 + NADPH.

  • Cellular localization

    Mitochondrion matrix.

  • Database links

    • Alternative names

      • Malate dehydrogenase antibody
      • Malic enzyme 3 antibody
      • Malic enzyme 3, NADP(+) dependent, mitochondrial antibody
      • NADP ME antibody
      • NADPME antibody
      • Pyruvic malic carboxylase antibody
      see all

    Images

    • Western blot - Anti-ME3 antibody [EPR10378] (ab172972)
      Western blot - Anti-ME3 antibody [EPR10378] (ab172972)
      All lanes : Anti-ME3 antibody [EPR10378] (ab172972) at 1/5000 dilution (purified)

      Lane 1 : Human fetal kidney lysates
      Lane 2 : Mouse heart lysates
      Lane 3 : Rat heart lysates
      Lane 4 : Mouse brain lysates
      Lane 5 : Rat brain lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 67 kDa
      Observed band size: 67 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (ab172972)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (ab172972)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling ME3 with Purified ab172972 at 1:200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
    • Western blot - Anti-ME3 antibody [EPR10378] (ab172972)
      Western blot - Anti-ME3 antibody [EPR10378] (ab172972)
      All lanes : Anti-ME3 antibody [EPR10378] (ab172972) at 1/1000 dilution (unpurified)

      Lane 1 : Fetal brain lysate
      Lane 2 : Fetal kidney lysate
      Lane 3 : Fetal heart lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 67 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (ab172972)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (ab172972)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ME3 with unpurified ab172972 at 1/50 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (ab172972)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (ab172972)

      Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling ME3 with unpurified ab172972 at 1/50 dilution.

    • Anti-ME3 antibody [EPR10378] (ab172972)
      Anti-ME3 antibody [EPR10378] (ab172972)

    References (5)

    Publishing research using ab172972? Please let us know so that we can cite the reference in this datasheet.

    ab172972 has been referenced in 5 publications.

    • Hosokawa M  et al. Loss of RNA-Binding Protein Sfpq Causes Long-Gene Transcriptopathy in Skeletal Muscle and Severe Muscle Mass Reduction with Metabolic Myopathy. iScience 13:229-242 (2019). PubMed: 30870781
    • Lu YX  et al. ME1 Regulates NADPH Homeostasis to Promote Gastric Cancer Growth and Metastasis. Cancer Res 78:1972-1985 (2018). PubMed: 29654155
    • Dey P  et al. Genomic deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer. Nature 542:119-123 (2017). WB ; Human . PubMed: 28099419
    • Liu L  et al. Malic enzyme tracers reveal hypoxia-induced switch in adipocyte NADPH pathway usage. Nat Chem Biol 12:345-52 (2016). PubMed: 26999781
    • Hasan NM  et al. Mitochondrial malic enzyme 3 is important for insulin secretion in pancreatic ß-cells. Mol Endocrinol 29:396-410 (2015). PubMed: 25594249

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