The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
1/100 - 1/500.
(or 1/50 - 1/200 using a fluorescent secondary antibody).
Measles virus belongs to the Paramyxoviridae family within the Mononegavirales order.
The Fusion protein, as its name suggests, is responsible for membrane fusion, essential for penetration into the host cell and subsequent initiation of the virus replicative cycle. Biological activity of the fusion protein is generated by endoproteolytic cleavage of a precursor protein (F0) into a large F1 subunit and a smaller F2 subunit held together by disulfide bonds.
Virion membrane; Single-pass type I membrane protein (By similarity). Cell membrane.