Anti-Measles nucleoprotein antibody [2D7] (ab9882)
- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-Measles nucleoprotein antibody [2D7]
See all Measles nucleoprotein primary antibodies -
DescriptionMouse monoclonal [2D7] to Measles nucleoprotein
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Host speciesMouse
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Tested applicationsSuitable for: ELISA, WBmore details
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Immunogen
Recombinant measles NP
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Positive control
- recombinant measles NP
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General notes
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium azide
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone number2D7
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MyelomaNS0/1
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IsotypeIgG1
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab9882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/1000 - 1/10000. | |
| WB | 1/500 - 1/2000. Detects a band of approximately 52.5 kDa. |
Target
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RelevanceInvolved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest
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Cellular localizationVirion. Host cytoplasm.
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Alternative names
- Measles nucleocapsid protein antibody
- N antibody
- NP antibody
- Nucleocapsid protein antibody
Images
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![Western blot - Anti-Measles nucleoprotein antibody [2D7] (ab9882)](https://www.abcam.com/ps/products/9/ab9882/Images/ab9882_1.gif)
Western blot - Anti-Measles nucleoprotein antibody [2D7] (ab9882)Western blot of measles NP using monoclonal antibody 2D7 at a concentration of 0.5 µg/ml.
Lane 1 - prestained marker proteins
Lane 2 - recombinant measles NP.Western blot of measles NP using monoclonal antibody 2D7 at a concentration of 0.5 µg/ml.
Lane 1 - prestained marker proteins
Lane 2 - recombinant measles NP.
Protocols
References
This product has been referenced in:
- Lo MK et al. GS-5734 and its parent nucleoside analog inhibit Filo-, Pneumo-, and Paramyxoviruses. Sci Rep 7:43395 (2017). Read more (PubMed: 28262699) »
- Morin I & Schaeffer PM Combining RNA-DNA swapping and quantitative polymerase chain reaction for the detection of influenza A nucleoprotein. Anal Biochem 420:121-6 (2012). Read more (PubMed: 21971444) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"