Anti-Measles nucleoprotein antibody [2D7] (ab9882)

Submit an Abreview Q&A (3)Specific References (5)

Overview

  • Product name
    Anti-Measles nucleoprotein antibody [2D7]
    See all Measles nucleoprotein primary antibodies
  • Description
    Mouse monoclonal [2D7] to Measles nucleoprotein
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Immunogen

    Recombinant measles NP

  • Positive control
    • recombinant measles NP
  • General notes

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    PBS with 0.1% sodium azide
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    2D7
  • Myeloma
    NS0/1
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/10000.
WB 1/500 - 1/2000. Detects a band of approximately 52.5 kDa.

Target

  • Relevance
    Involved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest
  • Cellular localization
    Virion. Host cytoplasm.
  • Alternative names
    • Measles nucleocapsid protein antibody
    • N antibody
    • NP antibody
    • Nucleocapsid protein antibody

Images

  • Western blot - Anti-Measles nucleoprotein antibody [2D7] (ab9882)
    Western blot - Anti-Measles nucleoprotein antibody [2D7] (ab9882)

    Western blot of measles NP using monoclonal antibody 2D7 at a concentration of 0.5 µg/ml.
    Lane 1 - prestained marker proteins
    Lane 2 - recombinant measles NP.

    Western blot of measles NP using monoclonal antibody 2D7 at a concentration of 0.5 µg/ml.
    Lane 1 - prestained marker proteins
    Lane 2 - recombinant measles NP.

References

This product has been referenced in:
  • Lo MK  et al. GS-5734 and its parent nucleoside analog inhibit Filo-, Pneumo-, and Paramyxoviruses. Sci Rep 7:43395 (2017). Read more (PubMed: 28262699) »
  • Morin I & Schaeffer PM Combining RNA-DNA swapping and quantitative polymerase chain reaction for the detection of influenza A nucleoprotein. Anal Biochem 420:121-6 (2012). Read more (PubMed: 21971444) »

See all 5 Publications for this product

Publishing research using ab9882? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

10
Votes: Highest First

What is the molecular weight of the protein? What is the size of the orange marker in the marker lane?

Thank you for your enquiry. The molecular weight of the protein (measles nucleocapsid protein) is 52.5 kDa. The size of the orange marker protein is 70 kDa. Please contact us again if you have additional questions.

Do you have antibody for "Measles virus nucleocapsid"? I like to know whether Nucleoprotein and Nucleocapsids are same? If so which of your product is best cross reacted for western application among the following: ab9882, ab9397, ab9884, ab9885 .

Thank you for your enquiry about our Measles nucleoprotein antibodies. A nucleoprotein is defined as any of a group of complexes composed of protein and nucleic acid and found in the nuclei and cytoplasm of all living cells, as in chromatin and rib...

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BATCH NUMBER 89420 ORDER NUMBER 8940 DESCRIPTION OF THE PROBLEM my customer purchased this antibody twice within one month (dec 04 and jan 05). The first time ab9882 performed as expected, one single band at 62 kd (using lot no. 2005...

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Thank you for the information that you have provided and I'm sorry to hear that your customer is experiencing difficulty with ab9882. We don't have lot 200502 in stock anymore. This is the first complaint that we have received concerning this antibody ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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