Anti-Measles nucleoprotein antibody [2D7] (ab9882)

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Key features and details

  • Mouse monoclonal [2D7] to Measles nucleoprotein
  • Suitable for: ELISA, WB
  • Reacts with: Species independent
  • Isotype: IgG1

Overview

  • Product name

    Anti-Measles nucleoprotein antibody [2D7]
    See all Measles nucleoprotein primary antibodies

  • Description

    Mouse monoclonal [2D7] to Measles nucleoprotein

  • Host species

    Mouse

  • Tested applications

    Suitable for: ELISA, WBmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Recombinant measles NP

  • Positive control

    • recombinant measles NP

  • General notes

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.1% Sodium azide
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    2D7

  • Myeloma

    NS0/1

  • Isotype

    IgG1

  • Light chain type

    unknown

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/10000.
WB 1/500 - 1/2000. Detects a band of approximately 52.5 kDa.

Target

  • Relevance

    Involved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest

  • Cellular localization

    Virion. Host cytoplasm.

  • Alternative names

    • Measles nucleocapsid protein antibody
    • N antibody
    • NP antibody
    • Nucleocapsid protein antibody

Images

  • Western blot - Anti-Measles nucleoprotein antibody [2D7] (ab9882)
    Western blot - Anti-Measles nucleoprotein antibody [2D7] (ab9882)

    Western blot of measles NP using monoclonal antibody 2D7 at a concentration of 0.5 µg/ml.
    Lane 1 - prestained marker proteins
    Lane 2 - recombinant measles NP.

    Western blot of measles NP using monoclonal antibody 2D7 at a concentration of 0.5 µg/ml.
    Lane 1 - prestained marker proteins
    Lane 2 - recombinant measles NP.

References

Publishing research using ab9882? Please let us know so that we can cite the reference in this datasheet.

ab9882 has been referenced in 5 publications.

  • Lo MK  et al. GS-5734 and its parent nucleoside analog inhibit Filo-, Pneumo-, and Paramyxoviruses. Sci Rep 7:43395 (2017). PubMed: 28262699
  • Morin I & Schaeffer PM Combining RNA-DNA swapping and quantitative polymerase chain reaction for the detection of influenza A nucleoprotein. Anal Biochem 420:121-6 (2012). PubMed: 21971444
  • Morin I  et al. IgG-detection devices for the Tus-Ter-lock immuno-PCR diagnostic platform. Analyst 136:4815-21 (2011). PubMed: 21980595
  • Kurihara N  et al. Contributions of the measles virus nucleocapsid gene and the SQSTM1/p62(P392L) mutation to Paget's disease. Cell Metab 13:23-34 (2011). IHC ; Human . PubMed: 21195346
  • Zvirbliene A  et al. Mapping of B cell epitopes in measles virus nucleocapsid protein. Arch Virol 152:25-39 (2007). PubMed: 16944047

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

What is the molecular weight of the protein? What is the size of the orange marker in the marker lane?

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Answer

Thank you for your enquiry. The molecular weight of the protein (measles nucleocapsid protein) is 52.5 kDa. The size of the orange marker protein is 70 kDa. Please contact us again if you have additional questions.

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Question

Do you have antibody for "Measles virus nucleocapsid"? I like to know whether Nucleoprotein and Nucleocapsids are same? If so which of your product is best cross reacted for western application among the following: ab9882, ab9397, ab9884, ab9885 .

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Answer

Thank you for your enquiry about our Measles nucleoprotein antibodies. A nucleoprotein is defined as any of a group of complexes composed of protein and nucleic acid and found in the nuclei and cytoplasm of all living cells, as in chromatin and ribosomes, and in viruses. A nucleocapsid is a unit of viral structure, consisting of a capsid (protein coat) with the enclosed nucleic acid; some simple viruses are naked nucleocapsids, while in others the nucleocapsids form part of a more complex structure. According to the database Expasy.org (http://www.expasy.org/cgi-bin/get-entries?KW=Viral%20nucleoprotein),a viral nucleocapsid protein is a synonym of a viral nucleoprotein therefore indeed they are the same. I have looked at the datasheets of the four antibodies you are interested in and I would recommend looking in particular at the datasheets of ab9882 and ab9397 as both have been tested in WB and therefore are guaranteed to work in this application. Ab9884 also works in WB but at a lower dilution so you will need to use more antibody. Ab9885 has not been tested in WB, only in ICC so is not guaranteed to work in WB. I hope this information will help you choose a suitable antibody for your experiments, please do not hesitate to contact me again if I can be of further help,

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Question

BATCH NUMBER 89420 ORDER NUMBER 8940 DESCRIPTION OF THE PROBLEM my customer purchased this antibody twice within one month (dec 04 and jan 05). The first time ab9882 performed as expected, one single band at 62 kd (using lot no. 200502). the second time (lot no. 89420) the antibody recognizes a totally different band (smaller). they performed the same experiment several times with exact same samples and got exact the same bad results. SAMPLE osteoclast precursor cells from TRAP-MVNP mice. PRIMARY ANTIBODY ab9882 lot 200502 and 89420 SECONDARY ANTIBODY na DETECTION METHOD na POSITIVE AND NEGATIVE CONTROLS USED na ANTIBODY STORAGE CONDITIONS according to instruction on datasheet SAMPLE PREPARATION same sample. AMOUNT OF PROTEIN LOADED 10 mg ELECTROPHORESIS/GEL CONDITIONS never changed since the first time TRANSFER AND BLOCKING CONDITIONS na HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? na ADDITIONAL NOTES we need a replacement vial from lot 200509

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Answer

Thank you for the information that you have provided and I'm sorry to hear that your customer is experiencing difficulty with ab9882. We don't have lot 200502 in stock anymore. This is the first complaint that we have received concerning this antibody and it's a possibility that the second vial that your customer received had gone off during shipping. I can offer you a free of charge replacement vial - please let me know.

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