Anti-Measles nucleoprotein antibody [2F3] (ab106292)
Key features and details
- Mouse monoclonal [2F3] to Measles nucleoprotein
- Suitable for: ICC/IF, ICC, WB, ELISA
- Isotype: IgG1
Overview
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Product name
Anti-Measles nucleoprotein antibody [2F3]
See all Measles nucleoprotein primary antibodies -
Description
Mouse monoclonal [2F3] to Measles nucleoprotein -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, ICC, WB, ELISAmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Recombinant Nucleoprotein
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2F3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent dilution.
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ICC |
1/100 - 1/500.
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WB |
1/200 - 1/1000. Predicted molecular weight: 58 kDa.
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ELISA |
1/2000 - 1/10000.
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Notes |
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ICC/IF
Use at an assay dependent dilution. |
ICC
1/100 - 1/500. |
WB
1/200 - 1/1000. Predicted molecular weight: 58 kDa. |
ELISA
1/2000 - 1/10000. |
Target
-
Relevance
Involved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest -
Cellular localization
Virion. Host cytoplasm. -
Database links
- Entrez Gene: 1489804 Other species
- SwissProt: P04851 Other species
- SwissProt: Q9WMB5 Other species
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Alternative names
- Measles nucleocapsid protein antibody
- N antibody
- NP antibody
- Nucleocapsid protein antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab106292 has been referenced in 3 publications.
- Zhou D et al. Establishment of an efficient reverse genetic system of Mumps virus S79 from cloned DNA. World J Pediatr 15:499-505 (2019). PubMed: 31456156
- Böröcz K et al. Development of a robust and standardized immunoserological assay for detection of anti-measles IgG antibodies in human sera. J Immunol Methods N/A:N/A (2018). PubMed: 30056035
- Wang Y et al. Enhancement of safety and immunogenicity of the Chinese Hu191 measles virus vaccine by alteration of the S-adenosylmethionine (SAM) binding site in the large polymerase protein. Virology 518:210-220 (2018). PubMed: 29525671