Anti-Measles nucleoprotein antibody [2F3] (ab106292)
Key features and details
- Mouse monoclonal [2F3] to Measles nucleoprotein
- Suitable for: ICC/IF, ICC, WB, ELISA
- Isotype: IgG1
Overview
-
Product name
Anti-Measles nucleoprotein antibody [2F3]
See all Measles nucleoprotein primary antibodies -
Description
Mouse monoclonal [2F3] to Measles nucleoprotein -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, ICC, WB, ELISAmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Recombinant Nucleoprotein
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2F3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent dilution.
|
|
| ICC |
1/100 - 1/500.
|
|
| WB |
1/200 - 1/1000. Predicted molecular weight: 58 kDa.
|
|
| ELISA |
1/2000 - 1/10000.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent dilution. |
|
ICC
1/100 - 1/500. |
|
WB
1/200 - 1/1000. Predicted molecular weight: 58 kDa. |
|
ELISA
1/2000 - 1/10000. |
Target
-
Relevance
Involved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest -
Cellular localization
Virion. Host cytoplasm. -
Database links
- Entrez Gene: 1489804 Other species
- SwissProt: P04851 Other species
- SwissProt: Q9WMB5 Other species
-
Alternative names
- Measles nucleocapsid protein antibody
- N antibody
- NP antibody
- Nucleocapsid protein antibody
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab106292 has been referenced in 3 publications.
- Zhou D et al. Establishment of an efficient reverse genetic system of Mumps virus S79 from cloned DNA. World J Pediatr 15:499-505 (2019). PubMed: 31456156
- Böröcz K et al. Development of a robust and standardized immunoserological assay for detection of anti-measles IgG antibodies in human sera. J Immunol Methods N/A:N/A (2018). PubMed: 30056035
- Wang Y et al. Enhancement of safety and immunogenicity of the Chinese Hu191 measles virus vaccine by alteration of the S-adenosylmethionine (SAM) binding site in the large polymerase protein. Virology 518:210-220 (2018). PubMed: 29525671