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    measles-nucleoprotein-antibody-2f3-ab106292.pdf

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Microbiology Organism Virus RNA Virus single stranded RNA negative strand virus Measles
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Anti-Measles nucleoprotein antibody [2F3] (ab106292)

  • Datasheet
  • SDS
Submit a review Submit a question References (3)

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Key features and details

  • Mouse monoclonal [2F3] to Measles nucleoprotein
  • Suitable for: ICC/IF, ICC, WB, ELISA
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Measles nucleoprotein antibody [2F3]
    See all Measles nucleoprotein primary antibodies
  • Description

    Mouse monoclonal [2F3] to Measles nucleoprotein
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, ICC, WB, ELISAmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Recombinant Nucleoprotein

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer

    Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    2F3
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • single stranded RNA negative strand virus
    • Measles

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab106292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent dilution.
ICC
1/100 - 1/500.
WB
1/200 - 1/1000. Predicted molecular weight: 58 kDa.
ELISA
1/2000 - 1/10000.
Notes
ICC/IF
Use at an assay dependent dilution.
ICC
1/100 - 1/500.
WB
1/200 - 1/1000. Predicted molecular weight: 58 kDa.
ELISA
1/2000 - 1/10000.

Target

  • Relevance

    Involved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest
  • Cellular localization

    Virion. Host cytoplasm.
  • Database links

    • Entrez Gene: 1489804 Other species
    • SwissProt: P04851 Other species
    • SwissProt: Q9WMB5 Other species
    • Alternative names

      • Measles nucleocapsid protein antibody
      • N antibody
      • NP antibody
      • Nucleocapsid protein antibody

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab106292? Please let us know so that we can cite the reference in this datasheet.

    ab106292 has been referenced in 3 publications.

    • Zhou D  et al. Establishment of an efficient reverse genetic system of Mumps virus S79 from cloned DNA. World J Pediatr 15:499-505 (2019). PubMed: 31456156
    • Böröcz K  et al. Development of a robust and standardized immunoserological assay for detection of anti-measles IgG antibodies in human sera. J Immunol Methods N/A:N/A (2018). PubMed: 30056035
    • Wang Y  et al. Enhancement of safety and immunogenicity of the Chinese Hu191 measles virus vaccine by alteration of the S-adenosylmethionine (SAM) binding site in the large polymerase protein. Virology 518:210-220 (2018). PubMed: 29525671

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