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  1. Link

    measles-nucleoprotein-antibody-3e1-ab9397.pdf

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Microbiology Organism Virus RNA Virus single stranded RNA negative strand virus Measles
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Anti-Measles nucleoprotein antibody [3E1] (ab9397)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (5)

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Western blot - Anti-Measles nucleoprotein antibody [3E1] (ab9397)

    Key features and details

    • Mouse monoclonal [3E1] to Measles nucleoprotein
    • Suitable for: WB
    • Reacts with: Species independent
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-Measles nucleoprotein antibody [3E1]
      See all Measles nucleoprotein primary antibodies
    • Description

      Mouse monoclonal [3E1] to Measles nucleoprotein
    • Host species

      Mouse
    • Specificity

      Reacts with measles NP. Does not cross-react with mumps NP. Recognises both native and recombinant yeast-derived measles NP.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Recombinant full length protein corresponding to Measles virus Measles nucleoprotein.

    • Positive control

      • recombinant measles NP
    • General notes

      This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR210126-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.2
      Preservative: 0.1% Sodium azide
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      3E1
    • Myeloma

      NS0/1
    • Isotype

      IgG1
    • Light chain type

      unknown
    • Research areas

      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • single stranded RNA negative strand virus
      • Measles

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab9397 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000 - 1/5000.
    Notes
    WB
    1/1000 - 1/5000.

    Target

    • Relevance

      Involved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest
    • Cellular localization

      Virion. Host cytoplasm.
    • Alternative names

      • Measles nucleocapsid protein antibody
      • N antibody
      • NP antibody
      • Nucleocapsid protein antibody

    Images

    • Western blot - Anti-Measles nucleoprotein antibody [3E1] (ab9397)
      Western blot - Anti-Measles nucleoprotein antibody [3E1] (ab9397)

      Immunoblot of measles NP using monoclonal antibody 3E1.
      Antibody 3E1 at a concentration of 0.5 µg/ml.
      Lane 1 - prestained marker proteins
      lane 2 - recombinant measles NP.

      Immunoblot of measles NP using monoclonal antibody 3E1. Antibody 3E1 at a concentration of 0.5 µg/ml. Lane 1 - prestained marker proteins lane 2 - recombinant measles NP.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab9397? Please let us know so that we can cite the reference in this datasheet.

    ab9397 has been referenced in 5 publications.

    • Sun Q  et al. TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation. J Bone Miner Res 29:90-102 (2014). PubMed: 23794264
    • Davis ME  et al. Antagonism of the phosphatase PP1 by the measles virus V protein is required for innate immune escape of MDA5. Cell Host Microbe 16:19-30 (2014). WB . PubMed: 25011105
    • Wang FM  et al. Measles virus nucleocapsid protein, a key contributor to Paget's disease, increases IL-6 expression via down-regulation of FoxO3/Sirt1 signaling. Bone 53:269-76 (2013). PubMed: 23262029
    • Ito M  et al. Measles virus nonstructural C protein modulates viral RNA polymerase activity by interacting with host protein SHCBP1. J Virol 87:9633-42 (2013). PubMed: 23804634
    • Sharma LB  et al. Contribution of matrix, fusion, hemagglutinin, and large protein genes of the CAM-70 measles virus vaccine strain to efficient growth in chicken embryonic fibroblasts. J Virol 83:11645-54 (2009). WB . PubMed: 19726516

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Do you have antibody for "Measles virus nucleocapsid"? I like to know whether Nucleoprotein and Nucleocapsids are same? If so which of your product is best cross reacted for western application among the following: ab9882, ab9397, ab9884, ab9885 .

    Read More

    Abcam community

    Verified customer

    Asked on Dec 28 2005

    Answer

    Thank you for your enquiry about our Measles nucleoprotein antibodies. A nucleoprotein is defined as any of a group of complexes composed of protein and nucleic acid and found in the nuclei and cytoplasm of all living cells, as in chromatin and ribosomes, and in viruses. A nucleocapsid is a unit of viral structure, consisting of a capsid (protein coat) with the enclosed nucleic acid; some simple viruses are naked nucleocapsids, while in others the nucleocapsids form part of a more complex structure. According to the database Expasy.org (http://www.expasy.org/cgi-bin/get-entries?KW=Viral%20nucleoprotein),a viral nucleocapsid protein is a synonym of a viral nucleoprotein therefore indeed they are the same. I have looked at the datasheets of the four antibodies you are interested in and I would recommend looking in particular at the datasheets of ab9882 and ab9397 as both have been tested in WB and therefore are guaranteed to work in this application. Ab9884 also works in WB but at a lower dilution so you will need to use more antibody. Ab9885 has not been tested in WB, only in ICC so is not guaranteed to work in WB. I hope this information will help you choose a suitable antibody for your experiments, please do not hesitate to contact me again if I can be of further help,

    Read More

    Abcam Scientific Support

    Answered on Dec 29 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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