Anti-Measles nucleoprotein antibody [3E1] (ab9397)
Key features and details
- Mouse monoclonal [3E1] to Measles nucleoprotein
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG1
Overview
-
Product name
Anti-Measles nucleoprotein antibody [3E1]
See all Measles nucleoprotein primary antibodies -
Description
Mouse monoclonal [3E1] to Measles nucleoprotein -
Host species
Mouse -
Specificity
Reacts with measles NP. Does not cross-react with mumps NP. Recognises both native and recombinant yeast-derived measles NP. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein corresponding to Measles virus Measles nucleoprotein.
-
Positive control
- recombinant measles NP
-
General notes
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR210126-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3E1 -
Myeloma
NS0/1 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000.
|
Notes |
---|
WB
1/1000 - 1/5000. |
Target
-
Relevance
Involved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest -
Cellular localization
Virion. Host cytoplasm. -
Alternative names
- Measles nucleocapsid protein antibody
- N antibody
- NP antibody
- Nucleocapsid protein antibody
Images
-
Immunoblot of measles NP using monoclonal antibody 3E1.
Immunoblot of measles NP using monoclonal antibody 3E1. Antibody 3E1 at a concentration of 0.5 µg/ml. Lane 1 - prestained marker proteins lane 2 - recombinant measles NP.
Antibody 3E1 at a concentration of 0.5 µg/ml.
Lane 1 - prestained marker proteins
lane 2 - recombinant measles NP.
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab9397 has been referenced in 5 publications.
- Sun Q et al. TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation. J Bone Miner Res 29:90-102 (2014). PubMed: 23794264
- Davis ME et al. Antagonism of the phosphatase PP1 by the measles virus V protein is required for innate immune escape of MDA5. Cell Host Microbe 16:19-30 (2014). WB . PubMed: 25011105
- Wang FM et al. Measles virus nucleocapsid protein, a key contributor to Paget's disease, increases IL-6 expression via down-regulation of FoxO3/Sirt1 signaling. Bone 53:269-76 (2013). PubMed: 23262029
- Ito M et al. Measles virus nonstructural C protein modulates viral RNA polymerase activity by interacting with host protein SHCBP1. J Virol 87:9633-42 (2013). PubMed: 23804634
- Sharma LB et al. Contribution of matrix, fusion, hemagglutinin, and large protein genes of the CAM-70 measles virus vaccine strain to efficient growth in chicken embryonic fibroblasts. J Virol 83:11645-54 (2009). WB . PubMed: 19726516