• Product name

    Anti-Measles nucleoprotein antibody [3E1]
    See all Measles nucleoprotein primary antibodies
  • Description

    Mouse monoclonal [3E1] to Measles nucleoprotein
  • Host species

  • Specificity

    Reacts with measles NP. Does not cross-react with mumps NP. Recognises both native and recombinant yeast-derived measles NP.
  • Tested applications

    Suitable for: WB, Indirect ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Recombinant full length protein.

  • Positive control

    • recombinant measles NP
  • General notes

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR210126-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

  • Clone number

  • Myeloma

  • Isotype

  • Light chain type

  • Research areas


Our Abpromise guarantee covers the use of ab9397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000.
Indirect ELISA 1/1000 - 1/10000.


  • Relevance

    Involved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest
  • Cellular localization

    Virion. Host cytoplasm.
  • Alternative names

    • Measles nucleocapsid protein antibody
    • N antibody
    • NP antibody
    • Nucleocapsid protein antibody


  • Immunoblot of measles NP using monoclonal antibody 3E1.
    Antibody 3E1 at a concentration of 0.5 µg/ml.
    Lane 1 - prestained marker proteins
    lane 2 - recombinant measles NP.

    Immunoblot of measles NP using monoclonal antibody 3E1. Antibody 3E1 at a concentration of 0.5 µg/ml. Lane 1 - prestained marker proteins lane 2 - recombinant measles NP.


This product has been referenced in:

  • Sun Q  et al. TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation. J Bone Miner Res 29:90-102 (2014). Read more (PubMed: 23794264) »
  • Davis ME  et al. Antagonism of the phosphatase PP1 by the measles virus V protein is required for innate immune escape of MDA5. Cell Host Microbe 16:19-30 (2014). WB . Read more (PubMed: 25011105) »
See all 5 Publications for this product

Customer reviews and Q&As


Thank you for your enquiry about our Measles nucleoprotein antibodies. A nucleoprotein is defined as any of a group of complexes composed of protein and nucleic acid and found in the nuclei and cytoplasm of all living cells, as in chromatin and ribosomes, and in viruses. A nucleocapsid is a unit of viral structure, consisting of a capsid (protein coat) with the enclosed nucleic acid; some simple viruses are naked nucleocapsids, while in others the nucleocapsids form part of a more complex structure. According to the database Expasy.org (http://www.expasy.org/cgi-bin/get-entries?KW=Viral%20nucleoprotein),a viral nucleocapsid protein is a synonym of a viral nucleoprotein therefore indeed they are the same. I have looked at the datasheets of the four antibodies you are interested in and I would recommend looking in particular at the datasheets of ab9882 and ab9397 as both have been tested in WB and therefore are guaranteed to work in this application. Ab9884 also works in WB but at a lower dilution so you will need to use more antibody. Ab9885 has not been tested in WB, only in ICC so is not guaranteed to work in WB. I hope this information will help you choose a suitable antibody for your experiments, please do not hesitate to contact me again if I can be of further help,

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