Anti-Measles phosphoprotein antibody [9H4] (ab43820)

Submit a review Q&A (3)References (2)

Overview

  • Product name
    Anti-Measles phosphoprotein antibody [9H4]
  • Description
    Mouse monoclonal [9H4] to Measles phosphoprotein
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Recombinant phosphoprotein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    9H4
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/10000.
WB 1/200 - 1/1000. Predicted molecular weight: 70 kDa.
ICC/IF 1/100 - 1/500.

Target

  • Relevance
    Measles virus belongs to the Paramyxoviridae family within the Mononegavirales order. Measles phosphoprotein is an essential component of the RNA polymerase and the nascent chain assembly complex. The non-segmented, single stranded, negative sense RNA genome of the virus is encapsidated by the nucleoprotein (N) to form a helical nucleocapsid. This ribonucleoproteic complex is the substrate for both transcription and replication. The RNA-dependent RNA polymerase binds to the nucleocapsid template via its co-factor, the phosphoprotein (P).
  • Alternative names
    • measles virus antibody
    • subacute sclerose panencephalitis virus antibody

References

This product has been referenced in:
  • Chung HK  et al. Tunable and reversible drug control of protein production via a self-excising degron. Nat Chem Biol 11:713-20 (2015). Read more (PubMed: 26214256) »
  • Krumm SA  et al. The measles virus nucleocapsid protein tail domain is dispensable for viral polymerase recruitment and activity. J Biol Chem 288:29943-53 (2013). WB . Read more (PubMed: 24003217) »
See all 2 Publications for this product

Publishing research using ab43820? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

I am working with a recombinant measles phosphoprotein and have introduced a couple of mutations. I am emailing to inquire the sequence for the epitope for this antibody to determine if it will be detected in WB with these introduced mutations.

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Answer

The monoclonal antibody ab43820 is raised against full-length recombinant phosphoprotein, but the single epitope it recognizes has not been mapped, so we cannot predict whether the mutations you have introduced will interfere with detection by ab43820. Chances are they will not, at least by western blotting, if they are single point mutations rather than large deletions.

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Question

Dear Technical Team,




Hope this e-mail finds you well!





Kindly advise if Anti-Measles phosphoprotein antibody [9H4] (ab43820)is targeted against the phosphorylated form or the unphosphorylated form of measles phosphoprotein.


Looking forward towards your valued response on it.



With Best Regards,

Read More
Answer

Thank you for your inquiry.

Please excuse the delay in my answer. I was waiting for more in formation from the source of this antibody.

They now confirmed that ab43820 binds to the phosphorylated protein only.

The un-phosphorylated protein is not recognized by this antibody.

I hope this information is helpful and wish you good luck with your research.

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Question

kindly provide me the following information required by our customer regarding ab43820

Please confirm whether this antibody- anti-measles phosphoprotein antibody (9H4) ab43820 - is targeted against the phosphorylated form or the unphosphorylated form of measles phosphoprotein.
Looking forward towards your response.

Best Regards.

Read More
Answer

Thank you for contacting us.

The antibody ab43820 is against phophorylated form of protein. I would suggest other antibodiers in case customer is interested in detecting unphosphorylated form of measles.

ab23974; ab106292; ab101015; ab9885

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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