Recombinant
RabMAb

Recombinant Anti-Meckelin antibody [EPR17187] (ab202062)

Submit a review Submit a question

Overview

  • Product name

    Anti-Meckelin antibody [EPR17187]
    See all Meckelin primary antibodies

  • Description

    Rabbit monoclonal [EPR17187] to Meckelin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Meckelin aa 800-900. The exact sequence is proprietary.
    Database link: Q5HYA8

  • Positive control

    • WB: MCF7, HepG2, 293, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal heart and fetal kidney lysates; mouse brain, mouse kidney, mouse spleen, rat brain and rat kidney lysates. IHC-P: Human breast carcinoma, Human lung carcinoma, Human fetal kidney, mouse liver and rat kidney tissues. ICC/IF: HeLa and MCF7 cells. Flow Cyt: MCF7 cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17187

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab202062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).Can be blocked with Goat Anti-Rabbit IgG H&L (HRP) (ab97051).
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/1000.
Flow Cyt 1/450.

Target

  • Relevance

    Meckelin localizes to the primary cilium and to the plasma membrane. It functions in centriole migration to the apical membrane and formation of the primary cilium. Defects in the gene are a cause of Meckel syndrome type 3 (MKS3) and Joubert syndrome type 6 (JBTS6).

  • Cellular localization

    Cell membrane; Multi-pass membrane protein

  • Database links

    • Alternative names

      • JBTS6 antibody
      • Meckel syndrome type 3 protein antibody
      • MKS3 antibody
      • NPHP11 antibody
      • TMEM67 antibody
      • TNEM67 antibody
      • Transmembrane protein 67 antibody
      see all

    Images

    • Western blot - Anti-Meckelin antibody [EPR17187] (ab202062)
      Western blot - Anti-Meckelin antibody [EPR17187] (ab202062)
      All lanes : Anti-Meckelin antibody [EPR17187] (ab202062) at 1/10000 dilution

      Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
      Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 112 kDa
      Observed band size: 112 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Meckelin antibody [EPR17187] (ab202062)
      Western blot - Anti-Meckelin antibody [EPR17187] (ab202062)
      All lanes : Anti-Meckelin antibody [EPR17187] (ab202062) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 112 kDa
      Observed band size: 112 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Meckelin antibody [EPR17187] (ab202062)
      Western blot - Anti-Meckelin antibody [EPR17187] (ab202062)
      All lanes : Anti-Meckelin antibody [EPR17187] (ab202062) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse kidney lysate
      Lane 3 : Mouse spleen lysate
      Lane 4 : Rat brain lysate
      Lane 5 : Rat kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 112 kDa
      Observed band size: 112 kDa


      Exposure time: 1 minute


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Meckelin antibody [EPR17187] (ab202062)
      Western blot - Anti-Meckelin antibody [EPR17187] (ab202062)
      All lanes : Anti-Meckelin antibody [EPR17187] (ab202062) at 1/1000 dilution

      Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
      Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 112 kDa
      Observed band size: 112 kDa


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Meckelin antibody [EPR17187] (ab202062)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Meckelin antibody [EPR17187] (ab202062)

      Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Meckelin with ab202062 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

      Cytoplasmic staining on Human breast carcinoma tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Meckelin antibody [EPR17187] (ab202062)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Meckelin antibody [EPR17187] (ab202062)

      Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Meckelin with ab202062 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

      Cytoplasmic staining on Human lung carcinoma tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Meckelin antibody [EPR17187] (ab202062)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Meckelin antibody [EPR17187] (ab202062)

      Immunohistochemical analysis of paraffin-embedded Human fetal kidney tissue labeling Meckelin with ab202062 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

      Cytoplasmic staining on Human fetal kidney tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Meckelin antibody [EPR17187] (ab202062)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Meckelin antibody [EPR17187] (ab202062)

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Meckelin with ab202062 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

      Cytoplasmic staining on mouse liver tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Meckelin antibody [EPR17187] (ab202062)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Meckelin antibody [EPR17187] (ab202062)

      Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Meckelin with ab202062 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

      Cytoplasmic staining on rat kidney tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Meckelin antibody [EPR17187] (ab202062)
      Immunocytochemistry/ Immunofluorescence - Anti-Meckelin antibody [EPR17187] (ab202062)

      Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Meckelin with ab202062 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

      Confocal image showing cytoplasmic staining on HeLa cell line.

      The nuclear counter stain is DAPI (blue).

      Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows:
      -ve control 1: ab202062 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-Meckelin antibody [EPR17187] (ab202062)
      Immunocytochemistry/ Immunofluorescence - Anti-Meckelin antibody [EPR17187] (ab202062)

      Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Meckelin with ab202062 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

      Confocal image showing cytoplasmic staining on MCF7 cell line.

      The nuclear counter stain is DAPI (blue).

      Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab202062 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    • Flow Cytometry - Anti-Meckelin antibody [EPR17187] (ab202062)
      Flow Cytometry - Anti-Meckelin antibody [EPR17187] (ab202062)

      Flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling Meckelin with ab202062 at 1/450 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    References

    ab202062 has not yet been referenced specifically in any publications.

    Publishing research using ab202062? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab202062.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com