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    mecp2-antibody-ab2828.pdf

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Epigenetics and Nuclear Signaling DNA methylation Methylated DNA Binding
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Anti-MeCP2 antibody (ab2828)

  • Datasheet
  • SDS
Reviews (27)Q&A (5)References (78)

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Immunocytochemistry/ Immunofluorescence - Anti-MeCP2 antibody (ab2828)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MeCP2 antibody (ab2828)
  • Immunocytochemistry/ Immunofluorescence - Anti-MeCP2 antibody (ab2828)
  • Western blot - Anti-MeCP2 antibody (ab2828)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MeCP2 antibody (ab2828)

Key features and details

  • Rabbit polyclonal to MeCP2
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-MeCP2 antibody [EPR23201-3] (ab253197)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-MeCP2 antibody
    See all MeCP2 primary antibodies
  • Description

    Rabbit polyclonal to MeCP2
  • Host species

    Rabbit
  • Specificity

    This antibody detects methyl CpG binding protein 2 (MeCP2).
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Non human primates, Amphibian, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Mouse MeCP2 aa 1-15.
    Sequence:

    MVAGMLGLREEKSED


    Database link: Q9Z2D6
    (Peptide available as ab4912)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC/IF: C6 and C2C12 cells IHC: Rat and mouse brain tissue WB: HeLa cells
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • Methylated DNA Binding
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Development

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human MeCP2 protein (His tag) (ab196070)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (3)
1/100 - 1/200.
IHC-P (1)
1/200 - 1/2000.
WB (11)
1/1500. Detects a band of approximately 56 kDa (predicted molecular weight: 52.4 kDa).

Recent lots of product ab2828 should detect the untruncated protein in human samples, 75 kDa band. Earlier lots detected the truncated version (55 kDa) in human and mouse samples. However in mouse samples, only a 55 kDa band is detected by all lots of the antibody. The difference between the earlier lots and the more recent lots is the antibodies were derived from different rabbits.

Notes
ICC/IF
1/100 - 1/200.
IHC-P
1/200 - 1/2000.
WB
1/1500. Detects a band of approximately 56 kDa (predicted molecular weight: 52.4 kDa).

Recent lots of product ab2828 should detect the untruncated protein in human samples, 75 kDa band. Earlier lots detected the truncated version (55 kDa) in human and mouse samples. However in mouse samples, only a 55 kDa band is detected by all lots of the antibody. The difference between the earlier lots and the more recent lots is the antibodies were derived from different rabbits.

Target

  • Function

    Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A.
  • Tissue specificity

    Present in all adult somatic tissues tested.
  • Involvement in disease

    Defects in MECP2 may be a cause of Angelman syndrome (AS) [MIM:105830]; also known as happy puppet syndrome. AS is a neurodevelopmental disorder characterized by severe mental retardation, absent speech, ataxia, sociable affect and dysmorphic facial features. AS and Rett syndrome have overlapping clinical features.
    Defects in MECP2 are the cause of mental retardation syndromic X-linked type 13 (MRXS13) [MIM:300055]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXS13 patients manifest mental retardation associated with other variable features such as spasticity, episodes of manic depressive psychosis, increased tone and macroorchidism.
    Defects in MECP2 are the cause of Rett syndrome (RTT) [MIM:312750]. RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Patients appear to develop normally until 6 to 18 months of age, then gradually lose speech and purposeful hand movements and develop microcephaly, seizures, autism, ataxia, intermittent hyperventilation, and stereotypic hand movements. After initial regression, the condition stabilizes and patients usually survive into adulthood.
    Defects in MECP2 may be the cause of susceptibility autism X-linked type 3 (AUTSX3) [MIM:300496]. AUTSX3 is a pervasive developmental disorder (PDD), prototypically characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age.
    Defects in MECP2 are the cause of encephalopathy neonatal severe due to MECP2 mutations (ENS-MECP2) [MIM:300673]. Note=The MECP2 gene is mutated in Rett syndrome, a severe neurodevelopmental disorder that almost always occurs in females. Although it was first thought that MECP2 mutations causing Rett syndrome were lethal in males, later reports identified a severe neonatal encephalopathy in surviving male sibs of patients with Rett syndrome. Additional reports have confirmed a severe phenotype in males with Rett syndrome-associated MECP2 mutations.
    Defects in MECP2 are the cause of mental retardation syndromic X-linked Lubs type (MRXSL) [MIM:300260]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSL patients manifest mental retardation associated with variable features. They include swallowing dysfunction and gastroesophageal reflux with secondary recurrent respiratory infections, hypotonia, mild myopathy and characteristic facies such as downslanting palpebral fissures, hypertelorism and a short nose with a low nasal bridge. Note=Increased dosage of MECP2 due to gene duplication appears to be responsible for the mental retardation phenotype.
  • Sequence similarities

    Contains 2 A.T hook DNA-binding domains.
    Contains 1 MBD (methyl-CpG-binding) domain.
  • Post-translational
    modifications

    Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.
  • Cellular localization

    Nucleus. Colocalized with methyl-CpG in the genome.
  • Target information above from: UniProt accession P51608 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4204 Human
    • Entrez Gene: 17257 Mouse
    • Entrez Gene: 29386 Rat
    • Omim: 300005 Human
    • SwissProt: P51608 Human
    • SwissProt: Q9Z2D6 Mouse
    • SwissProt: Q00566 Rat
    • Unigene: 200716 Human
    • Unigene: 568124 Human
    • Unigene: 131408 Mouse
    • Unigene: 414303 Mouse
    • Unigene: 9680 Rat
    see all
  • Alternative names

    • AUTSX 3 antibody
    • AUTSX3 antibody
    • DKFZp686A24160 antibody
    • Mbd 5 antibody
    • Mbd5 antibody
    • MECP 2 antibody
    • MeCP 2 protein antibody
    • MeCP-2 protein antibody
    • Mecp2 antibody
    • MECP2_HUMAN antibody
    • Methyl CpG binding protein 2 (Rett syndrome) antibody
    • Methyl CpG binding protein 2 antibody
    • Methyl-CpG-binding protein 2 antibody
    • MRX 16 antibody
    • MRX 79 antibody
    • MRX16 antibody
    • MRX79 antibody
    • MRXS 13 antibody
    • MRXS13 antibody
    • MRXSL antibody
    • PPMX antibody
    • RS antibody
    • RTS antibody
    • RTT antibody
    • WBP 10 antibody
    • WBP10 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-MeCP2 antibody (ab2828)
    Immunocytochemistry/ Immunofluorescence - Anti-MeCP2 antibody (ab2828)

    Immunocytochemistry/Immunoflorescence of C2C12 cells labeling Methyl CpG Binding Protein 2 (green) with ab2828 at a dilution of 1/200. Cells with primary antibody (right) compared to negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature.Cells were probed with polyclonal antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MeCP2 antibody (ab2828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MeCP2 antibody (ab2828)

    Immunohistochemistry analysis of mouse brain tissue staining MeCP2 with ab2828 (dilution 1/1000 in 3 % BSA-PBS). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab2828 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-MeCP2 antibody (ab2828)
    Immunocytochemistry/ Immunofluorescence - Anti-MeCP2 antibody (ab2828)

    Immunocytochemistry/Immunoflorescence of C6 cells labeling Methyl CpG Binding Protein 2 (green) with ab2828 at a dilution of 1/200. Cells with primary antibody (right) compared to negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature.Cells were probed with polyclonal antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

  • Western blot - Anti-MeCP2 antibody (ab2828)
    Western blot - Anti-MeCP2 antibody (ab2828)
    Western blot using ab2828 on HeLa cells. Western blot using ab2828 on HeLa cells.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MeCP2 antibody (ab2828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MeCP2 antibody (ab2828)

    Immunohistochemistry analysis of rat brain tissue staining MeCP2 with ab2828 (dilution 1/1000 in 3 % BSA-PBS). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab2828 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (78)

Publishing research using ab2828? Please let us know so that we can cite the reference in this datasheet.

ab2828 has been referenced in 78 publications.

  • Lee M  et al. Epigenetic regulation of p62/SQSTM1 overcomes the radioresistance of head and neck cancer cells via autophagy-dependent senescence induction. Cell Death Dis 12:250 (2021). PubMed: 33674559
  • Yao W  et al. Activation of BDNF by transcription factor Nrf2 contributes to antidepressant-like actions in rodents. Transl Psychiatry 11:140 (2021). PubMed: 33627628
  • Bacolla A  et al. Heritable pattern of oxidized DNA base repair coincides with pre-targeting of repair complexes to open chromatin. Nucleic Acids Res 49:221-243 (2021). PubMed: 33300026
  • Oyarzabal A  et al. Comprehensive Analysis of GABAA-A1R Developmental Alterations in Rett Syndrome: Setting the Focus for Therapeutic Targets in the Time Frame of the Disease. Int J Mol Sci 21:N/A (2020). PubMed: 31947619
  • Tong D  et al. MeCP2 facilitates breast cancer growth via promoting ubiquitination-mediated P53 degradation by inhibiting RPL5/RPL11 transcription. Oncogenesis 9:56 (2020). PubMed: 32483207
View all Publications for this product

Customer reviews and Q&As

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1-10 of 32 Abreviews or Q&A

Western blot abreview for Anti-MeCP2 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat primary cortical neurons)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Treatment
25uM ROS for 1hr
Specification
Rat primary cortical neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 12 2019

Western blot abreview for Anti-MeCP2 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary cortical neurons)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Treatment
50mM KCl or NaCl for 30min
Specification
Primary cortical neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 12 2019

Western blot abreview for Anti-MeCP2 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - nuclear (brain)
Gel Running Conditions
Reduced Denaturing (8% gel)
Loading amount
20 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 29 2017

Western blot abreview for Anti-MeCP2 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (lung)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
40 µg
Specification
lung
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 07 2017

RIP abreview for Anti-MeCP2 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
RIP
Immuno-precipitation step
Protein G
Detection step
Other
Sample
Mouse Cell lysate - whole cell (Neuroblastoma N2a cell)
Specification
Neuroblastoma N2a cell
Negative control
Immunoprecipitation without radioactive labeled DNA probe
Type
RIP without cross-linking
Duration of cross-linking step: 0 second(s)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Dec 15 2014

Immunoprecipitation abreview for Anti-MeCP2 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Rat Cell lysate - whole cell (Rat 20 DIV cortical neurons)
Specification
Rat 20 DIV cortical neurons
Treatment
25mM KCl
Total protein in input
20 µg
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Dec 15 2014

IHC - Wholemount abreview for Anti-MeCP2 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Embryo (Squashed polytene chromosomes dissected from 3rd i)
Specification
Squashed polytene chromosomes dissected from 3rd i
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Dec 15 2014

ChIP abreview for Anti-MeCP2 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Cell lysate - nuclear (5 DIV mouse cortical neurons)
Specification
5 DIV mouse cortical neurons
Negative control
BDNF exon II (which doesn't bind MeCP2)
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
BDNF exon IV (which binds MeCP2)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Dec 11 2014

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-MeCP2 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Rat cerebral cortex section)
Specification
Rat cerebral cortex section
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 11 2014

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-MeCP2 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Mouse hippocampus CA1)
Specification
Mouse hippocampus CA1
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 11 2014

1-10 of 32 Abreviews or Q&A

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