• Product name

  • Description

    Rabbit polyclonal to MED12
  • Host species

  • Tested applications

    Suitable for: IHC-P, ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human MED12 aa 2150-2177 (C terminal). Immunogen derived form sequence corresponding to amino acids 2150 - C-terminus.

  • Positive control

    • Whole cell lysate from HeLa, 293T and NIH 3T3 cells. IF: HeLa cell line



Our Abpromise guarantee covers the use of ab70842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 227 kDa.


  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
  • Tissue specificity

  • Involvement in disease

    Defects in MED12 are the cause of Opitz-Kaveggia syndrome (OKS) [MIM:305450]; also known as FG syndrome type 1 (FGS1) or FG syndrome (FGS). OKS is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.
    Defects in MED12 are the cause of Lujan-Fryns syndrome (LUJFRYS) [MIM:309520]; also known as X-linked mental retardation with marfanoid habitus. Clinically, Lujan-Fryns syndrome can be distinguished from Opitz-Kaveggia syndrome by tall stature, hypernasal voice, hyperextensible digits and high nasal root.
  • Sequence similarities

    Belongs to the Mediator complex subunit 12 family.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Activator recruited cofactor 240 kDa component antibody
    • Activator-recruited cofactor 240 kDa component antibody
    • ARC240 antibody
    • CAG repeat protein 45 antibody
    • CAGH45 antibody
    • HOPA antibody
    • KIAA0192 antibody
    • MED12 antibody
    • MED12_HUMAN antibody
    • Mediator complex subunit 12 antibody
    • Mediator of RNA polymerase II transcription subunit 12 antibody
    • OPA containing protein antibody
    • OPA-containing protein antibody
    • Thyroid hormone receptor associated protein complex 230 kDa component antibody
    • Thyroid hormone receptor-associated protein complex 230 kDa component antibody
    • TNRC11 antibody
    • Trap230 antibody
    • Trinucleotide repeat containing gene 11 protein antibody
    • Trinucleotide repeat-containing gene 11 protein antibody
    see all


  • All lanes : Anti-MED12 antibody (ab70842) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 227 kDa
    Observed band size: 238 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MED12 with ab70842 at 1/1000 (1µg/ml). Detection: DAB.
  • Detection of Human MED12 by Immunoprecipitation using Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70842 for IP at 3 µg/mg lysate. Subsequent WB detection was performed using ab70842 at 1 µg/ml.
  • ICC/IF image of ab70842 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70842, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Youn DY  et al. The subunit assembly state of the Mediator complex is nutrient-regulated and is dysregulated in a genetic model of insulin resistance and obesity. J Biol Chem 294:9076-9083 (2019). Read more (PubMed: 31028171) »
  • Shishkova E  et al. Global mapping of CARM1 substrates defines enzyme specificity and substrate recognition. Nat Commun 8:15571 (2017). WB . Read more (PubMed: 28537268) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Western blot
Loading amount
15 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12% Bis-Tris Gel, MES running buffer)
Mouse Tissue lysate - other (Mouse whole brain tissue lysate)
Mouse whole brain tissue lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 30 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Heat-inactivated normal donkey serum in 0.05% PBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Mouse Tissue sections (Mouse, whole brain sections, striatum, cortex)
Mouse, whole brain sections, striatum, cortex
Yes - Tween-20

Abcam user community

Verified customer

Submitted Oct 14 2013

Western blot
Human Cell lysate - nuclear (293)
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (6%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 01 2013

For licensing inquiries, please contact partnerships@abcam.com

Sign up