Recombinant Anti-MED12 antibody [BLR084G] (ab275962)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR084G] to MED12
- Suitable for: WB, IP, IHC-P, ICC
- Reacts with: Mouse, Human
Overview
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Product name
Anti-MED12 antibody [BLR084G]
See all MED12 primary antibodies -
Description
Rabbit monoclonal [BLR084G] to MED12 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICCmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human MED12 aa 2127-2177 (C terminal).
Database link: Q93074 -
Positive control
- WB: HeLa, A549, MCF-7, HepG2, K562, RKO, TCMK-1, and NIH/3T3 cell lysate. IP: HeLa cells. IHC-P: Breast carcinoma, renal carcinoma. ICC: HCT-116 cells.
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Borate buffered saline -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
BLR084G -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 243 kDa.
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IP |
Use at an assay dependent concentration.
20 µl/1 mg lysate |
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IHC-P |
1/100 - 1/500.
Epitope retrieval with citrate buffer pH6.0 is recommended |
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ICC |
1/100 - 1/500.
Epitope retrieval with citrate buffer pH6.0 is recommended. |
Notes |
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WB
1/1000. Predicted molecular weight: 243 kDa. |
IP
Use at an assay dependent concentration. 20 µl/1 mg lysate |
IHC-P
1/100 - 1/500. Epitope retrieval with citrate buffer pH6.0 is recommended |
ICC
1/100 - 1/500. Epitope retrieval with citrate buffer pH6.0 is recommended. |
Target
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Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in MED12 are the cause of Opitz-Kaveggia syndrome (OKS) [MIM:305450]; also known as FG syndrome type 1 (FGS1) or FG syndrome (FGS). OKS is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.
Defects in MED12 are the cause of Lujan-Fryns syndrome (LUJFRYS) [MIM:309520]; also known as X-linked mental retardation with marfanoid habitus. Clinically, Lujan-Fryns syndrome can be distinguished from Opitz-Kaveggia syndrome by tall stature, hypernasal voice, hyperextensible digits and high nasal root. -
Sequence similarities
Belongs to the Mediator complex subunit 12 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9968 Human
- Entrez Gene: 59024 Mouse
- Omim: 300188 Human
- SwissProt: Q93074 Human
- SwissProt: A2AGH6 Mouse
- Unigene: 409226 Human
- Unigene: 20873 Mouse
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Alternative names
- Activator recruited cofactor 240 kDa component antibody
- Activator-recruited cofactor 240 kDa component antibody
- ARC240 antibody
see all
Images
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Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
ab275962 used for IP at 20 µl/mg lysate. For blotting immunoprecipitated MED12, ab275962 was used at 1/1000 dilution. Chemiluminescence with an exposure time of 10 seconds.
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All lanes : Anti-MED12 antibody [BLR084G] (ab275962) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : K562 whole cell lysate
Lane 6 : RKO whole cell lysate
Lane 7 : TCMK-1 whole cell lysate
Lane 8 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 243 kDaChemiluminescence with an exposure time of 75 seconds.
Lower Panel: Rabbit anti-eEF2
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HCT-116 cells stained for MED12 using ab275962 at 1/500 dilution in immunocytochemistry.
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Paraffin embedded renal carcinoma tissue stained for MED12 using ab275962 at 1/500 dilution in immunohistochemical analysis.
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Paraffin embedded breast carcinoma tissue stained for MED12 using ab275962 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab275962 has not yet been referenced specifically in any publications.