Anti-MED13L antibody (ab87831)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-MED13L antibody
See all MED13L primary antibodies -
Description
Rabbit polyclonal to MED13L -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant
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Immunogen
Synthetic peptide within Human MED13L aa 550-600. The exact sequence is proprietary. (GeneID 23389).
Database link: NP_056150.1 -
Positive control
- Whole cell lysate from HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to MED13L immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab87831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 243 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. -
Tissue specificity
Highly expressed in brain (cerebellum), heart (aorta), skeletal muscle, kidney, placenta and peripheral blood leukocytes. Highly expressed in fetal brain. -
Involvement in disease
Defects in MED13L are a cause of transposition of the great arteries, dextro-looped (DTGA) [MIM:608808]. DTGA consists of complete inversion of the great vessels, so that the aorta incorrectly arises from the right ventricle and the pulmonary artery incorrectly arises from the left ventricle. This creates completely separate pulmonary and systemic circulatory systems, an arrangement that is incompatible with life. Patients often have atrial and/or ventricular septal defects or other types of shunting that allow some mixing between the circulations in order to support life minimally, but surgical intervention is always required.
Note=A chromosomal aberration involving MED13L is found in a patient with transposition of the great arteries, dextro-looped and mental retardation. Translocation t(12;17)(q24.1;q21). -
Sequence similarities
Belongs to the Mediator complex subunit 13 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23389 Human
- Entrez Gene: 360817 Rat
- Omim: 608771 Human
- SwissProt: Q71F56 Human
- Unigene: 603766 Human
- Unigene: 715095 Human
- Unigene: 41407 Rat
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Alternative names
- MD13L_HUMAN antibody
- MED13L antibody
- Mediator complex subunit 13 like antibody
see all
Images
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Western blot - Anti-MED13L antibody (ab87831)All lanes : Anti-MED13L antibody (ab87831) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Developed using the ECL technique.
Predicted band size: 243 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED13L antibody (ab87831)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MED13L with ab87831 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-MED13L antibody (ab87831)Detection of MED13L by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87831 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent WB detection. Chemiluminescence with an exposure time of 30 seconds.
Lane 1 : Anti-MED13L antibody (ab87831) at 1 µg/ml ((and used for IP at 3ug/mg lysate))
Lane 2 : Anti-MED13L antibody (ab87831) at 1 µg/ml (For IP: Rabbit anti-MED13L antibody only (which recognizes a downstream epitope))
Lane 3 : Anti-MED13L antibody (ab87831) at 1 µg/ml (For IP: Control IgG only)
Lane 1 : For WB: HeLa whole cell lysate at 50ug. For IP: HeLa whole cell lysate (1mg for IP, 20% of IP loaded)
Lane 2 : For WB: HeLa whole cell lysate at 15ug. For IP: HeLa whole cell lysate (1mg for IP, 20% of IP loaded)
Lane 3 : For WB: HeLa whole cell lysate at 5ug. For IP: HeLa whole cell lysate (1mg for IP, 20% of IP loaded)
Developed using the ECL technique.
Exposure time: 30 seconds
Datasheets and documents
References
This product has been referenced in:
- Tsai KL et al. A conserved Mediator-CDK8 kinase module association regulates Mediator-RNA polymerase II interaction. Nat Struct Mol Biol 20:611-9 (2013). WB ; Human . Read more (PubMed: 23563140) »
