Key features and details
- Rabbit polyclonal to MED13L
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MED13L antibody
See all MED13L primary antibodies
DescriptionRabbit polyclonal to MED13L
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant
Synthetic peptide within Human MED13L aa 550-600. The exact sequence is proprietary. (GeneID 23389).
Database link: NP_056150.1
- Whole cell lysate from HeLa cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to MED13L immobilized on solid support.
Our Abpromise guarantee covers the use of ab87831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 243 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
Tissue specificityHighly expressed in brain (cerebellum), heart (aorta), skeletal muscle, kidney, placenta and peripheral blood leukocytes. Highly expressed in fetal brain.
Involvement in diseaseDefects in MED13L are a cause of transposition of the great arteries, dextro-looped (DTGA) [MIM:608808]. DTGA consists of complete inversion of the great vessels, so that the aorta incorrectly arises from the right ventricle and the pulmonary artery incorrectly arises from the left ventricle. This creates completely separate pulmonary and systemic circulatory systems, an arrangement that is incompatible with life. Patients often have atrial and/or ventricular septal defects or other types of shunting that allow some mixing between the circulations in order to support life minimally, but surgical intervention is always required.
Note=A chromosomal aberration involving MED13L is found in a patient with transposition of the great arteries, dextro-looped and mental retardation. Translocation t(12;17)(q24.1;q21).
Sequence similaritiesBelongs to the Mediator complex subunit 13 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- MD13L_HUMAN antibody
- MED13L antibody
- Mediator complex subunit 13 like antibody
All lanes : Anti-MED13L antibody (ab87831) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Developed using the ECL technique.
Predicted band size: 243 kDa
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MED13L with ab87831 at 1/1000 (1µg/ml). Detection: DAB.
Detection of MED13L by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87831 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent WB detection. Chemiluminescence with an exposure time of 30 seconds.
Lane 1 : Anti-MED13L antibody (ab87831) at 1 µg/ml ((and used for IP at 3ug/mg lysate))
Lane 2 : Anti-MED13L antibody (ab87831) at 1 µg/ml (For IP: Rabbit anti-MED13L antibody only (which recognizes a downstream epitope))
Lane 3 : Anti-MED13L antibody (ab87831) at 1 µg/ml (For IP: Control IgG only)
Lane 1 : For WB: HeLa whole cell lysate at 50ug. For IP: HeLa whole cell lysate (1mg for IP, 20% of IP loaded)
Lane 2 : For WB: HeLa whole cell lysate at 15ug. For IP: HeLa whole cell lysate (1mg for IP, 20% of IP loaded)
Lane 3 : For WB: HeLa whole cell lysate at 5ug. For IP: HeLa whole cell lysate (1mg for IP, 20% of IP loaded)
Developed using the ECL technique.
Exposure time: 30 seconds
ab87831 has been referenced in 1 publication.
- Tsai KL et al. A conserved Mediator-CDK8 kinase module association regulates Mediator-RNA polymerase II interaction. Nat Struct Mol Biol 20:611-9 (2013). WB ; Human . PubMed: 23563140