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  1. Link

    med13l-antibody-ab87831.pdf

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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription Other
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Anti-MED13L antibody (ab87831)

  • Datasheet
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Western blot - Anti-MED13L antibody (ab87831)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED13L antibody (ab87831)
  • Immunoprecipitation - Anti-MED13L antibody (ab87831)

Key features and details

  • Rabbit polyclonal to MED13L
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Protein
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Recombinant Human MED13L protein (ab153226)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-MED13L antibody
    See all MED13L primary antibodies
  • Description

    Rabbit polyclonal to MED13L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant
  • Immunogen

    Synthetic peptide within Human MED13L aa 550-600. The exact sequence is proprietary. (GeneID 23389).
    Database link: NP_056150.1

  • Positive control

    • Whole cell lysate from HeLa cells
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to MED13L immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Mediator Complex

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human MED13L protein (ab153226)

Applications

Our Abpromise guarantee covers the use of ab87831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 243 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
  • Tissue specificity

    Highly expressed in brain (cerebellum), heart (aorta), skeletal muscle, kidney, placenta and peripheral blood leukocytes. Highly expressed in fetal brain.
  • Involvement in disease

    Defects in MED13L are a cause of transposition of the great arteries, dextro-looped (DTGA) [MIM:608808]. DTGA consists of complete inversion of the great vessels, so that the aorta incorrectly arises from the right ventricle and the pulmonary artery incorrectly arises from the left ventricle. This creates completely separate pulmonary and systemic circulatory systems, an arrangement that is incompatible with life. Patients often have atrial and/or ventricular septal defects or other types of shunting that allow some mixing between the circulations in order to support life minimally, but surgical intervention is always required.
    Note=A chromosomal aberration involving MED13L is found in a patient with transposition of the great arteries, dextro-looped and mental retardation. Translocation t(12;17)(q24.1;q21).
  • Sequence similarities

    Belongs to the Mediator complex subunit 13 family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q71F56 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23389 Human
    • Entrez Gene: 360817 Rat
    • Omim: 608771 Human
    • SwissProt: Q71F56 Human
    • Unigene: 603766 Human
    • Unigene: 715095 Human
    • Unigene: 41407 Rat
    • Alternative names

      • MD13L_HUMAN antibody
      • MED13L antibody
      • Mediator complex subunit 13 like antibody
      • Mediator complex subunit 13-like antibody
      • Mediator of RNA polymerase II transcription subunit 13-like antibody
      • THRAP2 antibody
      • Thyroid hormone receptor-associated protein 2 antibody
      • Thyroid hormone receptor-associated protein complex 240 kDa component-like antibody
      • TRAP240L antibody
      see all

    Images

    • Western blot - Anti-MED13L antibody (ab87831)
      Western blot - Anti-MED13L antibody (ab87831)
      All lanes : Anti-MED13L antibody (ab87831) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg/ml
      Lane 2 : HeLa whole cell lysate at 15 µg/ml
      Lane 3 : HeLa whole cell lysate at 5 µg/ml

      Developed using the ECL technique.

      Predicted band size: 243 kDa


      Exposure time: 3 minutes
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED13L antibody (ab87831)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED13L antibody (ab87831)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MED13L with ab87831 at 1/1000 (1µg/ml). Detection: DAB.
    • Immunoprecipitation - Anti-MED13L antibody (ab87831)
      Immunoprecipitation - Anti-MED13L antibody (ab87831)
      Detection of MED13L by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87831 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent WB detection. Chemiluminescence with an exposure time of 30 seconds.

      Lane 1 : Anti-MED13L antibody (ab87831) at 1 µg/ml ((and used for IP at 3ug/mg lysate))
      Lane 2 : Anti-MED13L antibody (ab87831) at 1 µg/ml (For IP: Rabbit anti-MED13L antibody only (which recognizes a downstream epitope))
      Lane 3 : Anti-MED13L antibody (ab87831) at 1 µg/ml (For IP: Control IgG only)

      Lane 1 : For WB: HeLa whole cell lysate at 50ug. For IP: HeLa whole cell lysate (1mg for IP, 20% of IP loaded)
      Lane 2 : For WB: HeLa whole cell lysate at 15ug. For IP: HeLa whole cell lysate (1mg for IP, 20% of IP loaded)
      Lane 3 : For WB: HeLa whole cell lysate at 5ug. For IP: HeLa whole cell lysate (1mg for IP, 20% of IP loaded)

      Developed using the ECL technique.

      Exposure time: 30 seconds

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab87831? Please let us know so that we can cite the reference in this datasheet.

    ab87831 has been referenced in 1 publication.

    • Tsai KL  et al. A conserved Mediator-CDK8 kinase module association regulates Mediator-RNA polymerase II interaction. Nat Struct Mol Biol 20:611-9 (2013). WB ; Human . PubMed: 23563140

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