Overview

  • Product name

  • Description

    Rabbit polyclonal to MED18
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment within Human MED18 (internal sequence). The exact sequence is proprietary.
    Database link: Q9BUE0

  • Positive control

    • WB: A549 whole cell lysate. IHC: Cal27 xenograft. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similarities

    Belongs to the Mediator complex subunit 18 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ20045 antibody
    • hMED 18 antibody
    • hMED18 antibody
    • Human Mediator of RNA Polymerase II Transcription antibody
    • MED 18 antibody
    • med18 antibody
    • MED18_HUMAN antibody
    • Mediator complex subunit 18 antibody
    • Mediator of RNA polymerase II transcription subunit 18 antibody
    • Mediator of RNA polymerase II transcription subunit 18 homolog antibody
    • OTTHUMP00000003645 antibody
    • p28b antibody
    • TRAP/mediator complex subunit p28b antibody
    see all

Images

  • Anti-MED18 antibody (ab228887) at 1/1000 dilution + A549 (Human lung carcinoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 24 kDa



    12% SDS-PAGE gel.

  • Paraffin-embedded Cal27 xenograft tissue stained for MED18 protein using ab228887 at 1/500 in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MED18 (green) using ab228887 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab228887 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228887.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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