Product nameAnti-MED18 antibody
See all MED18 primary antibodies
DescriptionRabbit polyclonal to MED18
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Catfish, Orangutan, Platypus
Synthetic peptide corresponding to Human MED18 (C terminal). A region between residue 150 and the C-terminus (residue 208) of human MED18 using the numbering given in entry NP_060108.1 (GeneID 54797).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab71556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 24 kDa.|
FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Sequence similaritiesBelongs to the Mediator complex subunit 18 family.
- Information by UniProt
- FLJ20045 antibody
- hMED 18 antibody
- hMED18 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MED18 with ab71556 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-MED18 antibody (ab71556) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Predicted band size: 24 kDa
Exposure time: 3 minutes
MED18 antibody (ab71556) at 3 µg/mg lysate, staining human MED18 in HeLa whole cell lysate by immunoprecipitation. 1 mg of HeLa whole cell lysate was used, 20% of IP loaded. Detection was by chemiluminescence with 30 second exposure.
ab71556 has not yet been referenced specifically in any publications.